The F-box protein SHORT PRIMARY ROOT modulates primary root meristem activity by targeting SEUSS-LIKE protein for degradation in rice  

作　　者：Nini Ma Nian Li Zhongmao Yu Chunli Chen Dao-Xiu Zhou Yu Zhao 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China [2]Institute of Plant Science Paris-Saclay(IPS2),CNRS,INRAE,University Paris-Saclay,Orsay 91405,France

出　　处：《Journal of Integrative Plant Biology》2023年第8期1937-1949,共13页植物学报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China (31671516 and 31970806)。

摘　　要：Root meristem activity is essential for root morphogenesis and adaptation,but the molecular mechanism regulating root meristem activity is not fully understood.Here,we identify an F-box family E3 ubiquitin ligase named SHORT PRIMARY ROOT(SHPR) that regulates primary root(PR)meristem activity and cell proliferation in rice.SHPR loss-of-function mutations impair PR elongation in rice.SHPR is involved in the formation of an SCF complex with the Oryza sativa SKP1-like protein OSK1/20.We show that SHPR interacts with Oryza sativa SEUSS-LIKE(OsSLK) in the nucleus and is required for OsSLK polyubiquitination and degradation by the ubiquitin 26S-proteasome system(UPS).Transgenic plants overexpressing OsSLK display a shorter PR phenotype,which is similar to the SHPR loss-of-function mutants.Genetic analysis suggests that SHPR promotes PR elongation in an OsSLK-dependent manner.Collectively,our study establishes SHPR as an E3 ubiquitin ligase that targets OsSLK for degradation,and uncovers a protein ubiquitination pathway as a mechanism for modulating root meristem activity in rice.

关 键 词：F-box protein primary root development RICE SLK(SEUSS-LIKE) ubiquitination 

分 类 号：S511[农业科学—作物学]