4-羟基香豆素-Ag复合材料的协同抑菌性能  

Synergistic bacteriostatic properties of 4-hydroxycoumarin-Ag composite

作　　者：梁犇 吴娟娟 郑锦丽 宋凤敏[1] 徐进 乔艳明[2] 郭少波 史娟[1] LIANG Ben;WU Juanjuan;ZHENG Jinli;SONG Fengmin;XU Jin;QIAO Yanming;GUO Shaobo;SHI Juan(School of Chemistry and Environmental Sciences,Shaanxi University of Technology,Hanzhong 723001,China;School of Biological Sciences and Engineering,Shaanxi University of Technology,Hanzhong 723001,China;Zhenba County Science and Technology Progress Promotion Center,Hanzhong 723001,China)

机构地区：[1]陕西理工大学化学与环境科学学院,汉中723001 [2]陕西理工大学生物科学与工程学院,汉中723001 [3]镇巴县科技进步促进中心,汉中723001

出　　处：《复合材料学报》2023年第8期4779-4791,共13页Acta Materiae Compositae Sinica

基　　金：陕西省科技厅项目(2020 ZDLGY11-02,2021 SF-382,2021 JQ-752);陕西理工大学秦巴生物资源与生态环境省部共建国家重点实验室项目(SXJ-2106);陕西理工大学科研一般项目(SLGKYXM2208);陕西省教育厅项目(22 JK0320)。

摘　　要：抗生素的滥用导致大量耐药菌出现,对卫生健康和社会经济造成严重威胁,因此,迫切需要开发新型抗菌剂来解决细菌耐药问题。本文以三氟醋酸银(CF3COOAg)为原料,采用化学还原法制备纳米银(Ag NPs)颗粒,并通过超声波辅助法结合4-羟基香豆素制备4-羟基香豆素-Ag NPs新型复合抑菌材料。利用TEM、XRD、XPS、UV-Vis、FTIR、Zeta电位及理论计算化学等进行表征。以革兰氏阴性菌大肠杆菌(E.coli)、革兰氏阳性菌金黄色葡萄球菌(S.aureus)和耐药菌沙门氏菌(T-Salmonella)为模式菌,研究4-羟基香豆素-Ag的协同抑菌活性及抑菌机制。抑菌性能结果显示:相比Ag NPs,复合材料对E.coli的抑菌效率提高了62.5%,对S.aureus提高了37.5%,对T-Salmonella提高了44.4%。复合材料在浓度为150μg/mL时,60 min内时对测试菌的抑菌率均可达99.9%。抑菌机制表明:复合材料可显著破坏细菌细胞壁,并进入细菌内部抑制细菌呼吸系统。该材料不仅具备单独无机抗菌剂和有机抗菌剂特有杀菌特性,两者协同且具备更强的抑菌活性,同时还可解决细菌耐药性问题,这可为抗生素的改性和新型抑菌剂的开发提供科学依据。The abuse of antibiotics leads to the emergence of a large number of drug-resistant bacteria,which poses a serious threat to health and social economy.Therefore,it is urgent to develop new antibacterial agents to solve the problem of bacterial drug resistance.In this paper,silver trifluoroacetate(CF3COOAg)as raw material,using chemical reduction method to prepare nano-silver(Ag NPs)particles,and ultrasonic assisted coordination with 4-hydroxycoumarin to prepare 4-hydroxycoumarin-Ag NPs new composite antibacterial material.TEM,XRD,XPS,UVVis,FTIR,Zeta potential and theoretical calculation were used for characterization.Gram-negative bacteria E.coli,Gram-positive bacteria S.aureus and drug-resistant bacteria T-Salmonella were used as model bacteria to study the synergistic bacteriostatic activity and bacteriostatic mechanism of 4-hydroxycoumarin-Ag.Compared with Ag NPs,the antibacterial efficiency of the composite material for E.coli,S.aureus and T-Salmonella has been improved62.5%,37.5%and 44.4%respectively.When the concentration of the composite material was 150μg/mL,the bacteriostatic rate of the tested bacteria could reach 99.9%within 60 min.The inhibition mechanism showed that the composite material could significantly destroy the bacterial cell wall and enter the bacterial interior to inhibit the bacterial respiratory system.The material not only has the unique bactericidal properties of inorganic antibacterial agent and organic antibacterial agent,but also has stronger antibacterial activity and can solve the problem of bacterial resistance,which can provide scientific basis for the modification of antibiotics and the development of new antibacterial agent.

关 键 词：协同抑菌 纳米银 4-羟基香豆素 抑菌机制 配位 

分 类 号：O62[理学—有机化学] TB333[理学—化学]