氟化氢铵接触工人骨代谢生物标志研究  

Study on biomarkers of bone metabolism in workers exposed to ammonium bifluoride

作　　者：郑光 孙赟 赵忠林 金惜雯 陈香 郑幼桥 张雪涛 ZHENG Guang;SUN Yun;ZHAO Zhonglin;JIN Xiwen;CHEN Xiang;ZHENG Youqiao;Zhang Xuetao(Shanghai Institute of Occupational Disease for Chemical Industry,Shanghai 200041,China;Jiangsu Taizhou Zetai Medical Examination Center,Taizhou,Jiangsu 253000,China)

机构地区：[1]上海市化工职业病防治院,上海200041 [2]江苏泰州泽泰体检中心,江苏泰州253000

出　　处：《职业卫生与应急救援》2023年第4期403-408,共6页Occupational Health and Emergency Rescue

基　　金：职业健康风险评估与国家职业卫生标准制定(131031109000160004);上海市第五轮公共卫生体系建设三年行动计划(2020—2022);环境与职业卫生学重点学科建设项目(GWV-10.1-XK12);上海市卫生健康地方标准预研制项目(2021WB26);上海市卫生健康委员会卫生健康政策研究课题(2023HP34)。

摘　　要：目的了解氟化氢铵接触人员尿氟和骨代谢生化指标变化情况,探讨氟化氢铵接触工人的骨代谢生物标志。方法于2021年12月,选择某半导体和液晶面板光罩生产企业氟化氢铵接触工人74名为接触组,临近区域某电子厂70名工人为对照组,比较两组工人尿氟、血清骨钙素、血清碱性磷酸酶、尿羟脯氨酸的差异。结果企业工作场所空气中氟化氢铵(以氟化氢计)浓度在<0.014~0.021 mg/m^(3)之间,低于国家职业接触限值。接触组工人班前尿氟值有7人超标,超标率为9.45%。接触组工人尿氟值与氟化氢外剂量值有正相关关系(r=0.332,P<0.05)。接触组工人尿氟质量浓度(0.69±0.77)mg/L,高于对照组的(0.47±0.17)mg/L,差异有统计学意义(P<0.05)。接触组工人血清骨钙素浓度(15.07±3.68)ng/mL,低于对照组的(16.51±4.51)ng/mL,差异有统计学意义(P<0.05)。接触组工人血清碱性磷酸酶浓度(76.99±14.99)U/L,对照组人工血清碱性磷酸酶浓度(75.03±22.35)U/L,两组差异无统计学意义(P>0.05)。接触组工人尿羟脯氨酸浓度(1134.01±145.34)μmol/L,高于对照组的(721.30±138.31)μmol/L,差异有统计学意义(P<0.01)。两组工人以不同尿氟值分组,>1.0 mg/L尿氟组工人尿羟脯氨酸值高于≤0.5 mg/L及>0.5~1.0 mg/L尿氟组工人(P<0.05)。结论尿氟、尿羟脯氨酸可能是氟化氢铵接触工人的敏感生物标志物。Objective To understand the urinary fluoride level and changes in bone biochemical metabolism indexes among workers exposed to ammonium bifluoride and to explore bone metabolism biomarkers in ammonium bifluoride-exposed workers.Methods Totally 74 workers occupationally exposed to ammonium bifluoride in a semiconductor and liquid crystal panel production enterprise(as an exposed group),as well as 70 workers in a nearby electronic factory(as a control group),were studied.The urinary fluoride,serum osteocalcin,serum alkaline phosphatase,and urine hydroxyproline were measured,and the differences between these two groups were compared.Results The airborne concentrations of hydrogen ammonium bifluoride(calculated as hydrogen fluoride) in the workplaces of the studied plant varied from non-detectable(lower than 0.014) to 0.021 mg/m^(3),which were lower than the national occupational exposure limit.Seven workers in the exposure group had excessive fluoride values in their pre-shift urine samples,with an excessive rate of 9.45%.There was a positive correlation between the urinary fluoride values of workers in the exposure group and the airborne concentration of hydrogen fluoride(r = 0.332,P<0.05).The urinary fluoride mass concentration of workers in the exposure group [(0.69 ±0.77) mg/L] was higher than that of the control group [(0.47 ± 0.17) mg/L],and the difference was statistically significant(P<0.05).The serum osteocalcin concentration of workers in the exposure group [(15.07 ± 3.68) ng/mL] was lower than that of the control group [(16.51 ± 4.51) ng/mL],and the difference was statistically significant(P<0.05).There was no significant difference between the serum alkaline phosphatase concentration [(76.99 ± 14.99) U/L] of workers in the exposure group and that of the control group [(75.03 ± 22.35) U/L](P>0.05).The urine hydroxyproline concentration of workers in the exposure group [(1 134.01 ± 145.34) μmol/L] was higher than that of the control group [(721.30 ±138.31) μmol/L],and the difference was statistic

关 键 词：尿氟 氟化氢铵 骨代谢 血清骨钙素 尿羟脯氨酸 碱性磷酸酶 生物标志 

分 类 号：R135[医药卫生—劳动卫生]