一步法制备PLA/PDA/Ag多孔抗菌纳米纤维膜及其促进伤口愈合作用研究  

Preparation of PLA/PDA/Ag Porous Antibacterial Nanofibrous Membrane by One-step and Their Effect on Promoting Wound Healing

作　　者：滕桂香 杨怡凡 侯苏童 姚慧 张春[3] TENG Guixiang;YANG Yifan;HOU Sutong;YAO Hui;ZHANG Chun(School of Biological and Pharmaceutical Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;WuXi PharmaTech,Tianjin 300457,China;School of Chemistry and Chemical Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China)

机构地区：[1]兰州交通大学生物与制药工程学院,兰州730070 [2]无锡药明康德新药开发股份有限公司,天津300457 [3]兰州交通大学化学化工学院,兰州730070

出　　处：《材料导报》2023年第18期240-245,共6页Materials Reports

基　　金：国家自然科学基金(52062025);甘肃省科技计划资助(22YF7NA149,22CX8GA062);甘肃省教育厅创新基金项目(2022A-032)。

摘　　要：有效控制伤口生物液不规则流出和防止伤口感染在促进伤口愈合和皮肤再生过程中存在巨大的挑战。本研究采用“静电纺丝+原位交联”的一步法制备了一种兼具渗出液吸附和伤口抗菌的PLA/PDA/Ag多孔纤维膜。通过对纤维膜化学组成、内部结构和力学性能等指标进行综合测试,并将其用于抗菌和伤口愈合实验,验证了纤维膜在创伤愈合中的实际应用。结果表明,PLA/PDA/Ag纤维膜的吸水率可达65.0%,接触角为72.35°,对伤口渗出液有明显的吸附和导流作用;该纤维膜对S.aureus和E.coli有明显抑菌作用,二者的抑菌圈直径分别为(25.4±0.6)mm和(28.7±0.9)mm;用于小鼠创伤实验时,第9 d小鼠皮肤创伤愈合度达到了95.3%,且未出现感染现象,伤口收缩率明显优于对照组。本研究制备的PLA/PDA/Ag纤维膜的亲水性、抗菌性和拉伸性可通过纺丝液配比进行调节,在伤口敷料设计和应用领域具有潜在的开发前景。Effective control the irregular flow of wound biological fluid and prevention of wound infection remain significant challenges in promoting wound healing and skin regeneration.In this study,a PLA/PDA/Ag nanofibrous membrane with exudate adsorption,wound antibacterial and mechanical properties was prepared by a one-step method of‘electrospinning+in-situ crosslinking’.After testing the chemical composition,internal structure and mechanical properties of the fibrous membrane,it was used in antibacteria and wound healing experiments to verify the practical application on wound healing.The results showed that the water absorption of PLA/PDA/Ag fibrous membrane can reached 65.0%and the water contact angle is 72.35°,which can contribute to the obvious adsorption and diversion effect on the wound exudate.The diameter of inhibition zones of S.ureus and E.coli were(25.4±0.6)mm and(28.7±0.9)mm,respectively when treated with the PLA/PDA/Ag fibrous membrane.The skin wound healing of mice reached 95.3%on the 9th day after treated with the membrane,with no infection occurred,and the wound contraction rate was significantly better than that of the control.The hydrophilicity,antibacterial property and stretchability of the PLA/PDA/Ag fibrous membrane prepared in this study can be adjusted by the addition ratio of DA,and then be used in different wound fields,which has certain application prospects in the field of wound accessory design.

关 键 词：静电纺丝 抗菌纤维 银纳米粒子 伤口愈合 

分 类 号：TQ340.64[化学工程—化纤工业] R318.08[医药卫生—生物医学工程]