Lactobacillus gasseri LA39 promotes hepatic primary bile acid biosynthesis and intestinal secondary bile acid biotransformation  

作　　者：Jun HU Qiliang HOU Wenyong ZHENG Tao YANG Xianghua YAN 

机构地区：[1]National Key Laboratory of Agricultural Microbiology,Hubei Hongshan Laboratory,Frontiers Science Center for Animal Breeding and Sustainable Production,College of Animal Sciences and Technology,Huazhong Agricultural University,Wuhan 430070,China [2]The Cooperative Innovation Center for Sustainable Pig Production,Wuhan 430070,China [3]Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology,Wuhan 430070,Chin

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2023年第8期734-748,共15页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the National Natural Science Foundation of China(Nos.31925037,31730090,and 32102499);the National Postdoctoral Program for Innovative Talents of China(No.BX20190133);the Postdoctoral Science Foundation of China(No.2019M662671);the Natural Science Foundation of Hubei Province(Nos.2022CFB358 and 2021CFA018).

摘　　要：A growing body of evidence has linked the gut microbiota to liver metabolism.The manipulation of intestinal microflora has been considered as a promising avenue to promote liver health.However,the effects of Lactobacillus gasseri LA39,a potential probiotic,on liver metabolism remain unclear.Accumulating studies have investigated the proteomic profile for mining the host biological events affected by microbes,and used the germ-free(GF)mouse model to evaluate host-microbe interaction.Here,we explored the effects of L.gasseri LA39 gavage on the protein expression profiles of the liver of GF mice.Our results showed that a total of 128 proteins were upregulated,whereas a total of 123 proteins were downregulated by treatment with L.gasseri LA39.Further bioinformatics analyses suggested that the primary bile acid(BA)biosynthesis pathway in the liver was activated by L.gasseri LA39.Three differentially expressed proteins(cytochrome P450 family 27 subfamily A member 1(CYP27A1),cytochrome P450 family 7 subfamily B member 1(CYP7B1),and cytochrome P450 family 8 subfamily B member 1(CYP8B1))involved in the primary BA biosynthesis pathway were further validated by western blot assay.In addition,targeted metabolomic analyses demonstrated that serum and fecalβ-muricholic acid(a primary BA),dehydrolithocholic acid(a secondary BA),and glycolithocholic acid-3-sulfate(a secondary BA)were significantly increased by L.gasseri LA39.Thus,our data revealed that L.gasseri LA39 activates the hepatic primary BA biosynthesis and promotes the intestinal secondary BA biotransformation.Based on these findings,we suggest that L.gasseri LA39 confers an important function in the gut‒liver axis through regulating BA metabolism.

关 键 词：Lactobacillus gasseri LA39 Liver Isobaric tags for relative and absolute quantitation(iTRAQ) Bile acid Germ-free mice 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]