腱糖蛋白C-pcDNA3.1重组质粒的构建及鉴定  

Construction and identification of recombinant plasmid Tenascin-C-pcDNA3.1

作　　者：李晓琴 李卓禧 宣焱 Li Xiaoqin;Li Zhuoxi;Xuan Yan(Department of Medical Laboratory,Shanxi Medical University Fenyang College,Fenyang Shanxi 032200,China;Department of Basic Medicine,Shanxi Medical University Fenyang College,Fenyang Shanxi 032200,China)

机构地区：[1]山西医科大学汾阳学院医学检验系,山西汾阳032200 [2]山西医科大学汾阳学院基础医学部,山西汾阳032200

出　　处：《中国药物与临床》2023年第1期10-12,I0002,I0003,共5页Chinese Remedies & Clinics

基　　金：山西省大学生创新创业训练项目(S202117114007)。

摘　　要：目的构建腱糖蛋白C(Tenascin-C)-pcDNA3.1真核表达质粒,并对重组质粒进行鉴定。方法体外合成细胞外基质糖蛋白Tenascin-C纤维蛋白原样球状体(FBG)结构域片段,双酶切法定向克隆至质粒载体pcDNA3.1,再进行连接产物的转化、挑菌、摇菌、质粒小提。酶切鉴定后,进行测序鉴定。结果酶切和测序结果显示Tenascin-C-pcDNA3.1质粒构建成功。结论成功构建了真核表达质粒Tenascin-C-pcDNA3.1,为后续转染及Tenascin-C FBG结构域在真核细胞中过表达奠定基础。Objective To construct and identify the recombinanteukaryotic expression plasmid Tenascin-C-pcDNA3.1.Methods The FBG domain fragment of extracellular matrix glycoprotein Tenascin-C was synthesized in vitro,and cloned into the plasmid vector pcDNA3.1 by double enzyme restriction digestion.The ligation prod-ucts were subjected to bacteria tranformation and selection,shaking,and plasmid extraction.After identification through enzyme digestion,sequence identification was performed.Results Enzyme digestion and sequencing showed successful construction of the plasmid Tenascin-C-pcDNA3.1.Conclusion In this study,the eukaryotic expression plasmid Tenascin-C-pcDNA3.1 was successfully constructed,which paves the way to subsequent trans-fection and overexpression of Tenascin-C FBG domain in eukaryotic cells.

关 键 词：腱糖蛋白 质粒 关节炎 类风湿 

分 类 号：Q789[生物学—分子生物学] R593.22[医药卫生—内科学]