Identification of blaIMI-mediated carbapenem-resistant Enterobacter from a duck farm in China  

作　　者：HUANG Hong-hao LU Yi-xing WU Su-juan MA Zhen-bao ZENG Dong-ping ZENG Zhen-ling 

机构地区：[1]National Laboratory of Safety Evaluation(Environmental Assessment)of Veterinary Drugs,College of Veterinary Medicine/China National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria/Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,College of Veterinary Medicine,South China Agricultural University [2]Guangdong Haid Group Co.,Ltd.,Guangzhou 511400,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第8期2500-2508,共9页农业科学学报（英文版）

基　　金：supported by the Foundation for Innovative Research Groups of the National Natural Science Foundation of China(32121004)。

摘　　要：Carbapenem-and colistin-resistant Enterobacter has been a clinical and therapy problem in recent years.Here,we report the carbapenem-and colistin-resistant Enterobacter harboring bla_(IMI) isolated from intestinal samples and the environment of a duck farm in China.Four bla_(IMI)-positive Enterobacter isolates were resistant to carbapenem and colistin.Three bla_(IMI) subtypes were detected in different molecular categories of Enterobacter.The detection of the various IMI producers highlights the diversity of carbapenemases in a duck farm.Whole-genome sequencing demonstrated the bla_(IMI) genes were present in chromosomes or plasmids in these strains.The conjugation experiment demonstrated the ability of bla_(IMI)-carrying plasmid to transmit horizontally.The molecular evolution characteristics were examined through comparative genetic analysis.The study demonstrated the presence of chromosomal and plasmid bla_(IMI) and the bla_(IMI)-carrying plasmid exhibits a horizontal transmission between Enterobacter and Escherichia coli C600.The similar genetic content was discovered between two bla_(IMI-16)-positive Enterobacter asburiae.In addition,a bla_(IMI-16)-carrying plasmid is an IncFII(Yp)plasmid,and a substantial amount of mobile genetic elements were identified around bla_(IMI-16).The IS-like elements and IncFII(Yp)plasmid are significant in the propagation of bla_(IMI).Our study provides evidence for the transmission of diverse bla_(IMI) genes in China and supplies additional reference data for bla_(IMI)-positive antimicrobialresistant Enterobacter.Routine surveys of bla_(IMI)-positive Enterobacter from animal-raising environments must be given more focus.

关 键 词：ENTEROBACTER antimicrobial resistance CARBAPENEMASES COLISTIN 

分 类 号：S852.61[农业科学—基础兽医学]