Ginsenoside-Rg1 combined with a conditioned medium from induced neuron-like hUCMSCs alleviated the apoptosis in a cell model of ALS through regulating the NF-κB/Bcl-2 pathway  

作　　者：HUANG Yu YANG Hui YANG Biying ZHENG Yu H OU Xiaomei CHEN Guiling ZHANG Wenqi ZENG Xiang DU Baoxin 

机构地区：[1]Labortory of Stem Cell Clinical Reaearch,The Second Clinical College of Guangzhou University of Chinese Medicine,Guangzhou 510006,China [2]Labortory of Stem Cell Biology and Chinese Medicine,The Second Afiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,China [3]Labortory of Stem Cell Biology and Chinese Medicine,Guangdong Provincial Academy of Chinese Medical Sciences,Guangzhou 510006,China [4]Labortory of Stem Cell Clinical Research,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510006,China [5]Department of Neurology,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510006,China

出　　处：《Chinese Journal of Natural Medicines》2023年第7期540-550,共11页中国天然药物（英文版）

基　　金：supported by the Scientific Research Project of the Traditional Chinese Medicine Bureau of Guangdong Province(Nos.20203002,20211188,and 20222082);the Special Funding for TCM Science and Technology Reasearch of Guangdong Provincial Hospital of Chinese Medicine(No.CMR-2017022-1001).

摘　　要：Amyotrophic lateral sclerosis(ALS)is a fatal neurodegenerative disease affecting both upper and lower motor neurons in the brain and spinal cord.One important aspect of ALS pathogenesis is superoxide dismutase 1(SOD1)mutant-mediated mitochondrial toxicity,leading to apoptosis in neurons.This study aimed to evaluate the neural protective synergistic effects of ginsenosides Rg1(G-Rg1)and conditioned medium(CM)on a mutational SOD1 cell model,and to explore the underlying mechanisms.We found that the contents of nerve growth factor,glial cell line-derived neurotrophic factor,and brain-derived neurotrophic factor significantly increased in CM after human umbilical cord mesenchymal stem cells(hUCMSCs)were exposed to neuron differentiation reagents for seven days.CM or G-Rg1 decreased the apoptotic rate of SOD1^(G93A)-NSC34 cell to a certain extent,but their combination brought about the least apoptosis,compared with CM or G-Rg1 alone.Further research showed that the anti-apoptotic protein Bcl-2 was upregulated in all the treatment groups.Proteins associated with mitochondrial apoptotic pathways,such as Bax,caspase 9(Cas-9),and cytochrome c(Cyt c),were downregulated.Furthermore,CM or G-Rg1 also inhibited the activation of the nuclear factor-kappa B(NF-κB)signaling pathway by reducing the phosphorylation of p65 and IκBa.CM/G-Rg1 or their combination also reduced the apoptotic rate induced by betulinic acid(BetA),an agonist of the NF-κB signaling pathway.In summary,the combination of CM and G-Rg1 effectively reduced the apoptosis of SOD1^(G93A)-NSC34 cells through suppresing the NF-κB/Bcl-2 sgaling pathway(Fig.1 is a graphcal representation of the abstract).

关 键 词：APOPTOSIS Amyotrophic lateral sclerosis Conditioned medium Ginsenoside-Rg1 Umbilical cord mesenchymal stem cells 

分 类 号：R965[医药卫生—药理学]