富含多种生物活性物质的桑黄菌筛选、纯种分离及鉴定  

Screening,isolation and identification of Inonotus hispidus riched in bioactive substances

作　　者：王文卷 亓正良 刘新利[1,2] 李正阔 陈若艳 盛晓芸 WANG Wenjuan;QI Zhengliang;LIU Xinli;LI Zhengkuo;CHEN Ruoyan;SHENG Xiaoyun(School of biological engineering,Qilu Universitry of Technology(Shandong Academy of Sciences),Jinan 250353,China;State Key Laboratory of bio-based materials and Green papermaking,Qilu Universitry of Technology(Shandong Academy of Sciences),Jinan 250353,China;XIXUN Fungi Research Institute,Yantai 265400,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生物工程学院,山东济南250353 [2]齐鲁工业大学(山东省科学院)生物基材料与绿色造纸国家重点实验室,山东济南250353 [3]喜蕈菌物研究院,山东烟台265400

出　　处：《齐鲁工业大学学报》2023年第4期73-80,共8页Journal of Qilu University of Technology

摘　　要：桑黄是一种具有悠久使用历史的药用真菌,含有的多糖、黄酮、三萜、多酚等活性物质,有很高的药用价值,现代药理学证实其所含的某些活性物质在抗肿瘤方面具有良好的效果。首先测定了11个不同来源的桑黄样品(SH-11、SH-12、SH-13、SH-14、SH-15、SH-16、SH-17、SH-18、SH-19、SH-20、SH-21)中的多糖、黄酮、三萜、多酚的含量,综合对比不同样品中的活性成分含量,筛选出一株富含上述活性成分的菌株SH-18,多酚、多糖、黄酮含量最高,三萜含量较高,分别达到了(19.58±1.044)、(96.35±9.01)、(17.08±0.29)、(9.1±0.14) mg/g,且该菌为野生来源。之后对其进行了分离纯化及分子鉴定,经鉴定SH-18为一株粗毛纤孔菌(Inonotus hispidus)。同时优化了活性物质的浸提方法,发现体积分数为70%乙醇对上述活性成分浸提效率较高。I.hispidus is a medicinal fungus with a long history of utilization.Its active substances such as polysaccharides,flavonoids,triterpenes and polyphenols have great medicinal value.Modern pharmacology has confirmed that some of these active substances have good effects on anti-tumor.In this study,the contents of polysaccharides,flavonoids,triterpenes and polyphenols in 11 samples of I.hispidus from different sources,(SH-11,SH-12,SH-13,SH-14,SH-15,SH-16,SH-17,SH-18,SH-19,SH-20 and SH-21)were determined.The SH-18 with the highest content of polyphenols,polysaccharides,flavonoids and high content of triterpenes was selected(19.58±1.044),(96.35±9.01),(17.08±0.29),(9.1±0.14)mg/g respectively.Finally,the SH-18 was identified as a strain of I.hispidus.The extraction method of the above active substances for I.hispidus was also optimized that 70%ethanol solution was beneficial to the extraction of the active substances.

关 键 词：桑黄 活性成分 分离鉴定 提取方法 

分 类 号：Q815[生物学—生物工程]