ROS(O_(2)^(-))信号在冬小麦根系细胞中的产生及传导机理  

ROS (O_(2)^(-)) Signal Generation and TransmissionMechanism in Winter Wheat Root Cells

作　　者：祁伟亮 孟建军[1,2,3] 张成 刘自成[1,2,3] 施万喜 杨虓[1,2,3] 乔岩 杨财容[4] 乔义林 李金玲[1] 陈思伟 姚来来[1] 赖守孝 张鹏 刘文辉 王婧 袁兰兰 QI Weiliang;MENG Jianjun;ZHANG Cheng;LIU Zicheng;SHI Wanxi;YANG Xiao;QIAO Yan;YANG Cairong;QIAO Yilin;LI Jinling;CHEN Siwei;YAO Lailai;LAI Shouxiao;ZHANG Peng;LIU Wenhui;WANG Jin;YUAN Lanlan(College of Agriculture and Forestry,Longdong University,Qingyang Gansu 745000 China;Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement and Industrialization,Qingyang Gansu 745000,China;Engineering Research Center for Germplasm Innovation and Application of Dryland Winter Wheat in Gansu Province,Qingyang Gansu 745000;College of Chemistry and Life Sciecne,Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources,Chengdu Normal University,Chengdu 611130,China)

机构地区：[1]陇东学院农林科技学院,甘肃庆阳745000 [2]陇东旱地作物种质改良及产业化协同创新中心,甘肃庆阳745000 [3]甘肃省旱地冬小麦种质创新与应用工程研究中心,甘肃庆阳745000 [4]成都师范学院化学与生命科学学院,特色园艺生物资源开发与利用四川省高校重点实验室,成都611130

出　　处：《西北农业学报》2023年第9期1325-1335,共11页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：庆阳市创新基地和人才计划(QY2021B-F003);陇东学院博士基金(901110050311);陇东学院创新创业项目(20221136,20221144);教育部产学合作协同育人项目(202102022080);国家自然科学基金(31960441)。

摘　　要：以冬小麦品种‘陇育5号’‘陇育8号’‘陇育10号’和品系‘陇育5-2’为材料,采用荧光原位杂交(FISH)技术以pSc119.2和pTa535为探针,明确4份冬小麦均属于六倍体材料(AABBDD,2n=42)。同时对冬小麦进行正常处理(25℃)、冷胁迫(4℃)和DPI+冷胁迫(4℃)处理并创新结合NBT法进行ROS定位。研究发现冬小麦在正常发芽过程中根尖顶端组织细胞和根毛区域检测到少量的ROS(O-2),推测少量的ROS(O-2)在冬小麦种子发芽及根系形成过程起到重要的调控作用。而在冷胁迫(4℃)处理环境下,‘陇育5号’和‘陇育5-2’(品系)的长势显著优于‘陇育8号’和‘陇育10号’,且冬小麦根系组织细胞区域发生ROS“大爆发”,其中在根尖顶端组织区域ROS信号最多,而在伸长组织区域ROS信号逐渐减少,推测ROS信号可能是一种动态传递的信号分子。同时还发现冬小麦维管束组织区域ROS信号积累较多,这也进一步暗示维管束组织可能是ROS信号产生和传导的主要通道。在DPI+冷胁迫(4℃)处理后,冬小麦根系组织细胞中的ROS信号明显减少,且以根伸长区和维管束组织细胞ROS消失最为明显,进一步说明NADPH酶是冬小麦在冷胁迫后ROS信号产生的主要来源途径。For this study,the winter wheat varieties‘Longyu5’‘Longyu8’‘Longyu10’and‘Longyu 5-2’were used as materials,we confirmed that all four winter wheat were hexaploid(AABBDD,2n=42)using PSC119.2 and pTa535 as probes in fluorescence in situ hybridization detection.Addtionally,we combined this method with NBT method to localize wheat treated with the normal temperature(25℃),cold stress(4℃),DPI+cold stress(4℃)and a control in ROS.The results showed that during normal germination of winter wheat,a small amount of ROS was detected in the apical tissue cells and root hairs,implying that this modest level of ROS may have significant impact on seed germination and root formation of winter wheat.The growth of‘Longyu 5’and‘Longyu 5-2’was significantly better growth than that of‘Longyu 8’and‘Longyu10’under cold stress(4℃).During the experiment,a“Big explosion”of ROS was also observed in the root tissue cell region of winter wheat,and the highest ROS signal was detected in the apical tissue region,which gradually decreased in the elongate tissue region,this suggests that the ROS signal may act as a dynamically transmitted signaling molecule.The results also showed that winter wheat had a high the accumulation of ROS in vascular bundle tissue,indicating that vascular bundle might be the main channel for ROS signal generation and conduction.Under treatment with DPI+cold stress(4℃)treatment,ROS in root tissue cells,particularly in root elongation area and vascular bundle tissue cells,decreased significantly.This,suggested that NADPH enzyme was the main source of ROS signal in winter wheat under cold stress.This study was of great significance not only for cold resistance breeding of gramineae crops and winter wheat,but also for the study of cold resistance signal transduction.Therefore,the finding provide new insights into the development of new varieties of cold-resistant crops.

关 键 词：冬小麦 活性氧 信号传导 冷胁迫 

分 类 号：S512.11[农业科学—作物学]