赤芍根腐病病原菌鉴定及生物学特性  

Identification and Biological Characteristics of PathogenSpecies of Root Rot on Paeonia veitchii Lynch

作　　者：马娅楠 李继平 郑果[1,2,3] 张金奎 马生彪[2] 惠娜娜 王立 张自强[1] MA Yanan;LI Jiping;ZHENG Guo;ZHANG Jinkui;MA Shengbiao;HUI Nana;WANG Li;ZHANG Ziqiang(College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China;Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;Tianshui Crop Pest Scientific Observation and Experiment Station of Ministry of Agriculture,Tianshui Gansu 741299,China)

机构地区：[1]甘肃农业大学植物保护学院,兰州730070 [2]甘肃省农业科学院植物保护研究所,兰州730070 [3]农业部天水作物有害生物科学观测试验站,甘肃天水741299

出　　处：《西北农业学报》2023年第9期1486-1494,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然科学基金(31560487)~~。

摘　　要：为明确甘肃省定西市一种新的赤芍根腐病病原菌及其生物学特性,采用常规组织分离法和孢子悬浮液离体接种法进行病原菌分离和致病性测定,基于形态学特征及内转录间隔区(Internal Transcribed Spacer, ITS)、核糖体大亚基(Ribosomal Large Subunit, LSU)和转录延伸因子1-α(Transcription elongation factor-1,TEF1-α)多基因序列分析进行病原菌鉴定,明确该病原菌为草莓垫壳孢(Coniella fragariae)。该病原菌生长最适温度为25℃,最适pH为6~7,全光照不利于菌丝生长和产孢,在PDA培养基上菌丝生长最快,在OA和黑麦培养基上产孢量最高,菌丝对乳糖和酵母膏的利用率最高。To clarify a new pathogen of root rot on Coniella fragariae and its biological characteristic in Dingxi of Gansu province,the pathogen was seperated and its pathogenicity was tested by the method of conventional tissue isolation and in vitro inoculation with spore suspension.The pathogenic fungus was identified to be C.fragariae based on morphology and ITS,LSU and TEF1-αpolygenic sequences.The optimal temperature for the growth of the pathogenic fungus is 25℃,the optimal pH value is 6-7,the full light is not conducive to hyphae growth and spore production,the hyphae growth is the fastest on PDA medium,the highest spore production on OA and rye medium,and the highest utilization rate of hyphae to lactose and yeast paste.

关 键 词：赤芍 根腐病 病原菌分离鉴定 草莓垫壳孢 生物学特性 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]