丹顶鹤卵壳孵化前后超微结构与元素含量研究  

Ultrastructure and element content of Red-crowned crane eggshell before and after hatching

作　　者：高忠燕 刘志超 陈善雨 杨志宏[2] GAO Zhong-yan;LIU Zhi-chao;CHEN Shan-yu;YANG Zhi-hong(Zhalong National Nature Reserve,Heilongjiang Qiqihar 161003,China;College of Life Science,Agriculture and Forestry,Qiqihar University,Heilongjiang Qiqihar 161006,China)

机构地区：[1]黑龙江省扎龙国家级自然保护区,黑龙江齐齐哈尔161003 [2]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《齐齐哈尔大学学报（自然科学版）》2023年第5期81-85,共5页Journal of Qiqihar University(Natural Science Edition)

基　　金：黑龙江省省属本科高校基本科研业务费面上项目(135409324)。

摘　　要：为比较丹顶鹤卵壳孵化前后超微结构与元素质量浓度差异性,利用扫描电子显微镜观察卵壳超微结构,采用电感偶合等离子体光谱仪检测卵壳化学元素,对孵化前(对照组,n=8)后(实验组,n=12)2组卵壳进行比较。孵化前后卵壳表面晶体层、栅栏锥体层和壳膜层超微结构均有明显差异,孵化后卵壳厚度小于孵化前(P﹤0.05);孵化后卵壳中有8种元素质量浓度(8/23)比孵化前卵壳显著减少(P﹤0.05)。结果表明,幼鸟在其卵壳内胚胎发育过程中,需要消耗卵壳中部分元素构成骨骼等器官组织,卵壳结构与功能随之发生相应变化。In order to compare the ultrastructure and element content of two groups red-crowned crane eggshells before hatching(n=8)and after hatching(n=12)were compared.The ultrastructure was observed by scanning electron microscope and the element content was detected by inductively paired plasma spectrometer.The results showed that the ultrastructure of surface layer of crystals,palisade cone layer and eggshell membrane were significantly different before and after hatching,the thickness of eggshell after hatching was less than that before hatching(P<0.05),and the contents of 8 elements(8/23)after hatching were significantly reduced than before hatching(P<0.05).In conclusion,during embryonic development,some elements in the eggshell were consumed to form organs such as bones,the structure and function of eggshell changed accordingly.

关 键 词：丹顶鹤 卵壳 超微结构 元素组成 

分 类 号：Q959.726[生物学—动物学]