马齿苋水提物对保育仔猪生长性能的影响及其机制  

Effect and Mechanism of Portulaca oleracea Extract on Growth Performance of Nursery Piglets

作　　者：张建臣 ZHANG Jian-chen(Shandong Vocational Animal Science and Veterinary College,Weifang 261061,China)

机构地区：[1]山东畜牧兽医职业学院,山东潍坊261061

出　　处：《中国兽医杂志》2023年第8期143-148,共6页Chinese Journal of Veterinary Medicine

基　　金：潍坊高新区2021年科技惠民计划(2021KJHM19)。

摘　　要：为了明确饲料中添加马齿苋提取物(Portulaca oleracea extract)对保育仔猪生长性能的影响及其作用机制,本试验采用水提干燥法制备马齿苋提取物,用不同剂量的提取物饲喂保育仔猪后,根据仔猪腹泻率、日增重、血液常规和血清生化指标评价马齿苋提取物对保育仔猪的安全性和作用效果,同时设立金霉素组和空白对照组;通过ELISA方法检测血清中生长激素(GH)、生长激素抑制激素(GHIH)和炎性细胞因子含量,分析马齿苋提取物的作用机制。结果显示,随着饲料中添加马齿苋提取物剂量的增加,仔猪腹泻率逐渐降低,但差异无统计学意义(P>0.05);每吨饲料中添加600 g或800 g马齿苋提取物可极显著提高仔猪的日增重(P<0.01),显著升高仔猪血清中总蛋白(TP)的含量(P<0.05),显著降低仔猪血清中尿素氮(BUN)和谷丙转氨酶(ALT)的含量(P<0.05),而对仔猪血液常规指标无显著影响(P>0.05);饲喂不同剂量马齿苋提取物后,仔猪血清中GHIH含量无显著变化(P>0.05),IL-6含量显著降低(P<0.05),每吨饲料中添加600 g或800 g马齿苋提取物可显著提高仔猪血清中GH含量(P<0.05),只有添加800 g马齿苋提取物可显著降低仔猪血清中TNF-α含量(P<0.05)。结果表明,在每吨饲料中添加600 g或800 g的马齿苋提取物可起到与金霉素一致的促生长效果,其可能是通过提高血清GH含量和降低炎症因子含量发挥作用。The present study investigated the effect of Portulaca oleracea extract on growth performance of nursery piglets,and explored the underlying mechanism of action.Portulaca oleracea extract was prepared by water extraction and drying method,nursery piglets were fed with different doses of extract,and the safety and effect of Portulaca oleracea extract on piglets were evaluated according to diarrhea rate,daily gain,blood routine and serum biochemical indexes,with chlortetracycline group and blank control group as controls.The mechanism of Portulaca oleracea extract was analyzed by detecting the levels of growth hormone(GH),growth hormone inhibitory hormone(GHIH)and inflammatory cytokines in the serum by ELISA kits.The results showed that the diarrhea rate of piglets decreased gradually with the increase of the dose of Portulaca oleracea extract in the feed,but the difference was not statistically significant(P>0.05);addition of 600 g or 800 g Portulaca oleracea extract per ton of feed significantly increased the daily gain of piglets(P<0.01),significantly increased the total protein(TP)content in serum of piglets(P<0.05),and significantly reduced the of urea nitrogen(BUN)and alanine aminotransferase(ALT)contents in serum of piglets(P<0.05),but had no significant effect on blood routine indexes of piglets(P>0.05).After feeding different doses of Portulaca oleracea extract,the GHIH content in serum of piglets did not change significantly(P>0.05),the serum IL-6 content decreased significantly(P<0.05);addition of 600 g or 800 g Portulaca oleracea extract per ton of feed significantly increased the serum GH content of piglets(P<0.05),addition of 800 g Portulaca oleracea extract per ton of feed significantly decreased the serum TNF-αcontent of piglets(P<0.05).Thus,the addition of 600 g or 800 g Portulaca oleracea extract per ton of feed could promote growth of nursery piglets similar to chlortetracycline leading to increased content of serum GH and reduced content of inflammatory factors.

关 键 词：马齿苋提取物 生长性能 激素含量 炎性因子 

分 类 号：S828[农业科学—畜牧学]