基于SSR标记初步定位小豆驯化相关性状QTL  

Mapping of QTL for Domestication-related Traits Using SSR Markers in Adzuki Bean

作　　者：贾龙 罗高玲[3] 陈燕华[3] 王素华[2] 陈红霖[2] 刘长友[4] 杜吉到[1] 王丽侠[2] JIA Long;LUO Gaoling;CHEN Yanhua;WANG Suhua;CHEN Hongling;LIU Changyou;DU Jidao;WANG Lixia(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing 163319;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081;Institute of Rice Research,Guangxi Academy of Agricultural Sciences,Nanning 530007;Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050035)

机构地区：[1]黑龙江八一农垦大学农学院,大庆163319 [2]中国农业科学院作物科学研究所,北京100081 [3]广西农业科学院水稻研究所,南宁530007 [4]河北省农林科学院粮油作物研究所,石家庄050035

出　　处：《植物遗传资源学报》2023年第5期1426-1434,共9页Journal of Plant Genetic Resources

基　　金：国家现代农业产业技术体系(CARS-08)。

摘　　要：炸荚和籽粒硬实性是野生小豆的主要特性,严重制约着野生小豆资源的利用,解析这些性状的遗传机理,对培育优良小豆新品种具有重要意义。本研究基于中红5号和野生小豆(Vigna angularis var.n ipponensis)杂交衍生的重组自交系,开展炸荚和籽粒硬实等驯化相关性状鉴定及SSR标记分析。表型分析显示荚皮卷曲数、炸荚率及籽粒硬实率均不符合典型正态分布。构建了包含111个SSR标记、11个连锁群的小豆遗传连锁图谱,总长3813.5 cM,标记间平均距离为34.35 cM。连锁分析共发掘到与2021年炸荚率和荚皮卷曲数相关QTL 19个,与2022年炸荚率及荚皮卷曲数相关QTL 13个,其中不同年份重复检测到的荚皮卷曲数相关QTL 2个,重复检测到的炸荚率相关QTL 3个。籽粒硬实相关QTL 4个,其中位于LG11的QTL分别与炸荚率及2022年荚皮卷曲数相关QTL重叠。本研究结果为进一步开展小豆驯化相关基因的精细定位及机理解析奠定了基础。Wild adzuki bean are important gene resources for genetic improvement of cultivars,however,pod shattering and hard seededness are classical characters of wild genotypes,and are disadvantage traits in breeding.In the present study,we used a recombination inbred line(RIL)population,developed from a cross between a cultivar and a wild genotype(Vigna angularis var.nipponensis),to identify QTL loci related with pod shattering and hard seededness using SSR markers.The results showed that the ratio of pod shattering(PS),the number of pod curling(NPC)and the ratio of hard seededness(HSN)were not fitful for normal distribution,but had continuous variation.Eleven linkage groups were constructed using 111 SSR markers with a total length of 3813.5 cM and 34.35 cM for intervals.A total of 19 QTLs were detected for PS and NPC in 2021 and 13 were detected in 2022.Two QTLs were repeatedly detected to be related with the NPC and 3 were with PS.There were 4 QTLs that related with the hard seededness and the one on LG11 were also repeatedly detected with PS in 2021 and 2022,and NPC in 2022.The present results provided insights for fine mapping of these domesticate-related traits and investigating the functional mechanisms.

关 键 词：小豆 SSR标记 遗传图谱 驯化性状 QTL 

分 类 号：S521[农业科学—作物学]