水稻加工和外观品质性状QTL鉴定  

Identification of QTL for Milling and Appearance Quality Traits in Rice(Oryza sativa L.)

作　　者：姚晓云 陈春莲[1] 熊运华[1] 黄永萍[1] 彭志勤[1] 刘进 尹建华[1] YAO Xiaoyun;CHEN Chunlian;XIONG Yunhua;HUANG Yongping;PENG Zhiqing;LIU Jin;YIN Jianhua(Rice Research Institute,Jiangxi Academy of Agricultural Sciences/National Engineering Laboratory for Rice(Nanchang)/Nanchang Sub-center,National Rice Improvement Center,Nanchang 330200,China)

机构地区：[1]江西省农业科学院水稻研究所/水稻国家工程研究中心(南昌)/国家水稻改良中心南昌分中心,南昌330200

出　　处：《中国水稻科学》2023年第5期507-517,共11页Chinese Journal of Rice Science

基　　金：江西现代农业科研协同创新专项(JXXTCXQN202208,JXXTCXQN202205);江西省水稻产业技术体系项目(JXARX-02);江西省青年基金资助项目(20202BABL215002,20212BAB215030)。

摘　　要：【目的】稻米加工与外观品质是影响水稻生产效益和商品价值的关键因素,发掘相关性状主效QTL有助于进一步阐明水稻品质性状的分子遗传机制,也可为水稻优质育种提供材料。【方法】以优质粳稻龙稻5号为母本和高产籼稻中优早8号为父本杂交衍生的重组自交系群体为试验材料,在3个环境条件下对稻米加工和外观品质进行性状比较和QTL分析。【结果】检测到37个与加工和外观品质相关的QTL,分布在12条染色体上,三个环境分别发现15、15和21个QTL,其中仅10个QTL在2个环境中稳定表达,2个QTL在3个环境中重复检测到。加工和外观品质相关QTL具有明显的遗传重叠效应,第2、6、7、10和11染色体上存在主效QTL簇,对加工和外观品质存在明显的调控作用。【结论】第7染色体RM1306−RM420区间存在一个新的QTL qMAQ7(qChlak7),该区间对垩白粒率和垩白度具有较强的调控效应;此外,上位性互作也是调控加工和外观品质的重要组成部分,主效QTL参与上位性互作效应。【Objective】Milling and appearance quality(MAQ)are the key factors affecting the production efficiency and commodity value of rice.Exploring the major QTLs for MAQ related traits help further clarify the molecular genetic mechanism of quality traits,which also provides genetic materials for high-quality breeding in rice.【Method】A recombinant inbred line(RIL)population derived from the cross between Longdao 5(high-quality japonica)and Zhongyouzao 8(high-yield indica)was used to identify QTLs for milling and appearance quality traits in multiple environments.【Result】Thirty-seven QTLs related to processing and appearance quality were detected on 12 chromosomes.15,15,and 21 QTLs were found in the three environments,respectively.Only 10 QTLs were stably expressed in two environments,and two QTLs were repeatedly detected in all the three environments.Processing and appearance quality related QTLs showed significant genetic overlap effects.In addition,QTLs of the milling and appearance quality had obvious genetic overlap effect.There were five major QTL clusters on the chromosomes 2,6,7,10 and 11,which had obvious regulatory effects on milling and appearance quality.【Conclusion】qMAQ7(qChlak7)is a novel pleiotropic QTL cluster on chromosome 7 between RM1306 and RM420,which exerts obvious regulatory effects on percentage of chalky grains and chalkiness degree.In addition,the epistatic effect plays an important role in the regulation of milling and appearance quality traits,and then the major effect QTL was involved in epistatic interaction effect.

关 键 词：水稻 加工品质 外观品质 QTL分析 qChalk7 

分 类 号：S511[农业科学—作物学]