针刺对哮喘大鼠肺功能及气道平滑肌痉挛的影响  

Effects of acupuncture on pulmonary functioonn a annod airway smooth muscle spasm in asthma rats

作　　者：时宜蓉 易蔚[2] 乔赟[1] 卓少元 张倩[1] 杨雪捷 梁韬[1] 凌希[1] SHI Yi-rong;YI Wei;QIAO Yun;ZHUO Shao-yuan;ZHANG Qian;YANG Xue-jie;LIANG Tao;LING Xi(College of Acupuncture-Moxibustion and Tuina,Guangxi University of CM,Nanning 530200,China;Graduate School,Guangxi University of CM,Nanning 530200,China;College of Basic Medicine,Guangxi University of CM,Nanning 530200,China)

机构地区：[1]广西中医药大学针灸推拿学院,南宁530200 [2]广西中医药大学研究生院,南宁530200 [3]广西中医药大学基础医学院,南宁530200

出　　处：《中国针灸》2023年第8期937-943,958,共8页Chinese Acupuncture & Moxibustion

基　　金：广西自然科学基金资助项目:2020GXNSFAA297021。

摘　　要：目的:观察针刺“孔最”“鱼际”对哮喘大鼠引喘潜伏期、肺功能及内皮素-1(ET-1)、金属硫蛋白-2(MT-2)表达的影响,探讨针刺缓解气道平滑肌痉挛、改善哮喘急性发作的作用机制。方法:将40只SPF级雄性SD大鼠随机分为正常组、模型组、药物组及针刺组,每组10只。除正常组外,其余3组均采用卵蛋白致敏法建立哮喘模型。药物组予沙丁胺醇雾化,针刺组针刺双侧“孔最”“鱼际”,均每日1次,共14 d。观察各组大鼠引喘潜伏期及肺功能,ELISA法检测血清及支气管肺泡灌洗液(BALF)ET-1及肿瘤坏死因子(TNF)-α含量,采用Masson染色观察气道形态,采用透射电镜观察气道平滑肌超微结构,采用实时荧光定量PCR及Western blot法检测肺组织ET-1、MT-2的mRNA及蛋白表达。结果:与正常组比较,模型组引喘潜伏期缩短(P<0.01),气道阻力(RL)升高、肺顺应性(Cdyn)降低(P<0.01,P<0.05);血清及BALF中ET-1及TNF-α含量升高(P<0.01);支气管周围可见胶原纤维及胶原沉积,气道平滑肌增厚;细胞损伤较重,线粒体肿胀;ET-1 mRNA及蛋白表达升高、MT-2 mRNA及蛋白表达降低(P<0.01)。与模型组比较,针刺组引喘潜伏期延长(P<0.05),RL降低、Cdyn升高(P<0.01,P<0.05)。与模型组比较,针刺组、药物组血清及BALF中ET-1、TNF-α含量降低(P<0.01,P<0.05);支气管周围胶原纤维及胶原沉积减少,气道平滑肌增厚程度减轻,细胞损伤较轻;ET-1 mRNA及蛋白表达降低、MT-2 mRNA及蛋白表达升高(P<0.01)。与药物组比较,针刺组MT-2 mRNA表达升高(P<0.05)。结论:针刺“孔最”“鱼际”可改善哮喘大鼠肺功能,松弛气道平滑肌痉挛,其作用机制可能与下调ET-1表达、上调MT-2表达有关。Objective To observe the effects of acupuncture at"Kongzui"(LU 6)and"Yuji"(LU 10)on the latent period of inducing asthma,pulmonary function and the expression of endothelin-1(ET-1)and metallothionein-2(MT-2)in asthma rats,and to explore the possible mechanism of acupuncture in alleviating airway smooth muscle spasm and improving the acute attack of asthma.Methods A total of 40 male SD rats of SPF-grade were randomly divided into a normal group,a model group,a medication group and an acupuncture group,10 rats in each group.Except for the normal group,ovalbumin sensitization method was used to establish the asthma model in the other 3 groups.Salbutamol nebulization was adopted in the medication group,while acupuncture was applied at bilateral"Kongzui"(LU 6)and"Yuji"(LU 10)in the acupuncture group.The intervention was given once a day for 14 days in the two groups.The latent period of inducing asthma and pulmonary function were observed,the levels of ET-1 and tumor necrosis factor(TNF)-αin serum and bronchoalveolar lavage fluid(BALF)were detected by ELISA method,the morphology of the airway was observed by Masson staining,the ultrastructure of the airway smooth muscle was observed by transmission electron microscopy,the mRNA and protein expression of ET-1 and MT-2 in lung tissue was detected by real-time PCR and Western blot methods.Results Compared with the normal group,in the model group,the latent period of inducing asthma was shortened(P<0.01);the airway resistance(RL)was increased while the dynamic compliance(Cdyn)was decreased(P<0.01,P<0.05);the levels of ET-1 and TNF-αin serum and BALF were increased(P<0.01);collagen fibers and collagen depositions were found around the bronchi,airway smooth muscle was thickened,the cell damage was severe and mitochondria were swollen;the mRNA and protein expression of ET-1 was increased while the mRNA and protein expression of MT-2 was decreased(P<0.01).Compared with the model group,in the acupuncture group,the latent period of inducing asthma was prolonged(P<0.05),the RL

关 键 词：哮喘 针刺 穴 孔最 穴 鱼际 气道平滑肌 内皮素-1 金属硫蛋白-2 

分 类 号：R245.31[医药卫生—针灸推拿学]