铁皇冠炭疽病病原鉴定及室内药剂筛选  

Pathogen Identification and Screening of Fungicides Against Microsorum pteropus Anthracnose Disease

作　　者：崔一平[1] 宋晓兵[1] 严洁春 黄峰 凌金锋[1] 彭埃天[1] CUI Yiping;SONG Xiaobing;YAN Jiechun;HUANG Feng;LING Jinfeng;PENG Aitian(Institute of Plant Protection,Guangdong Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou 510640;Huadu District Agricultural Technology Management Center of Guangzhou,Guangzhou 510813)

机构地区：[1]广东省农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广东省植物保护新技术重点实验室,广州510640 [2]广州市花都区农业技术管理中心,广州510813

出　　处：《中国农学通报》2023年第24期120-126,共7页Chinese Agricultural Science Bulletin

基　　金：广东省农村科技特派员项目“火龙果重要病虫害精准防控及品质提升技术示范及推广”(KTP20210317);广东省农业科学院协同创新中心项目“农业生物安全与农产品质量安全绿色植保技术与产品”(XT202211)。

摘　　要：为明确引起广东省肇庆市高要区铁皇冠炭疽病的病原种类并筛选出具有较好防治效果的药剂,采用组织分离法对铁皇冠炭疽病病原菌进行了分离纯化;结合形态学观察、多基因系统发育分析及柯赫氏法则对该病原菌进行了分类鉴定。同时采用菌丝生长速率法,选用42.4%唑醚氟酰胺WG、75%肟菌·戊唑醇WG、35%400g/L氟菌·戊唑醇SC、325 g/L苯甲·嘧菌酯SC、60%唑醚·代森联WG、10%苯醚甲环唑WG对该炭疽菌的室内毒力进行测定。结果显示,引起铁皇冠炭疽病的病原菌为果生炭疽菌(Colletotrichum fructicola),供试的6种杀菌剂对C.fructicola的菌丝生长均有一定的抑制作用,其中10%苯醚甲环唑水分散粒剂的抑制活性最强,其次为325 g/L苯甲·嘧菌酯悬浮剂、75%肟菌·戊唑醇水分散粒剂,以上3种杀菌剂均可推荐为田间防控药剂。In order to clarify the pathogen causing Microsorum pteropus anthracnose disease in Gaoyao district,Zhaoqing City,Guangdong Province,and to screen better effect fungicides against the disease indoor,the pathogen was obtained by using the tissue separation method.The representative isolates were verified according to Koch's rule.Combined with morphological characteristics and phylogenetic tree based on ITS,ACT,CAL and CHS-1 genes,the pathogen was identified,and the indoor toxicity of six fungicides(42.4%pyraclostrobin·metiram WG,75% trifloxystrobin·tebuconazole WG,35% 400 g/L fluopyram·tebuconazole SC,325 g/L difenoconazole · azoxystrobin SC,60% pyraclostrobin ·fluxapyroxad WG,10% difenoconazole WG)was determined by mycelia growth rate method.Colletotrichum fructicola was the pathogen causing anthracnose disease on Microsorum pteropus.The tested six fungicides had certain inhibitory effect on the mycelia growth of C.fructicola.Among them,10% difenoconazole WG had the strongest inhibitory activity with moderate inhibitory concentration(EC50) of 0.1083 μg/mL.Followed by 325 g/L difenoconazole· azoxystrobin SC and75% trifloxystrobin· tebuconazole WG,the above three fungicides can be recommended as field control agents.

关 键 词：广东省 铁皇冠 果生炭疽菌 柯赫氏法则 室内毒力 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]