响应面法优化消瘤藤总黄酮提取工艺及其抗氧化活性研究  

Research on the optimization of the extraction process and antioxidant activity of total flavonoids from Pileostegia tomentella by response surface methodology

作　　者：黎强 周颖 卢森华 LI Qiang;ZHOU Ying;LU Sen-hua(Yulin Food and Drug Inspection and Testing Center,Yulin537000,Guangxi,China;Yulin Engineering Research Center for Quality Inspection and Evaluation of Traditional Chinese Medicine Materials(Including Spices),Yulin537000,Guangxi,China)

机构地区：[1]玉林市食品药品检验检测中心,广西玉林537000 [2]玉林中药材(含香辛料)质量检测与评价工程技术研究中心,广西玉林537000

出　　处：《湖北农业科学》2023年第8期167-174,共8页Hubei Agricultural Sciences

基　　金：广西科技基地和人才专项(桂科AD22080002)。

摘　　要：采用单因素试验确定超声法提取消瘤藤总黄酮,以提取温度、乙醇体积分数、料液比三个因素做三水平Box-Behnken响应面法设计进行提取工艺优化,并计算其DPPH自由基的清除率和还原能力。结果表明,消瘤藤总黄酮最佳提取工艺条件为提取温度49℃、乙醇体积分数46%、料液比1∶66 g/mL,该条件下消瘤藤总黄酮得率为27.01 mg/g。不同产地的消瘤藤总黄酮含量存在显著差异。在测定的质量浓度中,消瘤藤总黄酮对DPPH自由基有较好的清除能力,其还原能力随总黄酮浓度的增大而增强。消瘤藤总黄酮的提取工艺稳定、操作简单、方法学良好,可用于其工业化大生产。Using a single factor experiment to determine the ultrasonic extraction of total flavonoids from Pileostegia tomentella,three-level Box-Behnken response surface methodology was used to optimize the extraction process with three factors of extraction temperature,ethanol volume fraction,and solid-liquid ratio,and the scavenging rate of DPPH free radicals and reducing ability were calculated.The results showed that the condition for the optimal extraction process of total flavonoids from Pileostegia tomentella was extraction temperature of 49℃,ethanol volume fraction of 46%,and solid-liquid ratio of 1∶66 g/mL.The total flavonoids yield from Pileostegia tomentella under the condition was 27.01 mg/g.There were significant differences in the total flavonoids content of Pileostegia tomentella in different origins.In the measured mass concentration,the total flavonoids from Pileostegia tomentella had a good scavenging ability to DPPH free radicals,and its reducing ability increased with the increase of total flavonoids concentration.The extraction process of the total flavonoids from Pileostegia tomentella was stable,the operation was simple,and the methodology was good,which could be used for its large-scale industrial production.

关 键 词：消瘤藤 总黄酮 响应面法 抗氧化 

分 类 号：R284.2[医药卫生—中药学]