青礞石抗乌头碱致大鼠室性心律失常作用及对心肌组织P38 MAPK表达的影响  

Treatment Effect of Qingmengshi(Chloriti Lapis)on Rats’Ventricular Arrhythmia Induced by Aconitine and Impact on P38 MAPK

作　　者：尚宜志 郭茂松 张守红 SHANG Yizhi;GUO Maosong;ZHANG Shouhong(Heilongjiang Academy of Chinese Medicine Sciences,Harbin,Heilongjiang 150036)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036

出　　处：《中国中医药科技》2023年第5期862-865,共4页Chinese Journal of Traditional Medical Science and Technology

基　　金：黑龙江省卫生健康委课题(2020-275)。

摘　　要：目的:观察青礞石对乌头碱致大鼠室性早搏(premature ventricular beats,PVC)、室颤(ventricular fibrillation,VF)、心脏停搏(cardiac arrest,SCA)的影响,并探讨其抗室性心律失常机制。方法:40只SD大鼠随机分为模型组、青礞石(低、中、高剂量)组,各药物组直肠纳药干预2周,于末次给药1 h后,20%乌拉坦(1 g/kg)腹腔麻醉,大鼠背位固定,由股静脉以5μg/min恒速泵入乌头碱20μg/kg,记录PVC、VF、SCA发生率、开始时间及乌头碱中毒剂量。通过蛋白质免疫印迹法检测心肌组织P38丝裂原激活蛋白激酶(MAPK)表达。结果:与模型组相比,青礞石高、中、低剂量组显著降低PVC、VF、SCA发生率,开始时间明显延后(P<0.05);与模型组比较,青礞石高、中、低剂量组乌头碱中毒剂量增加(P<0.05)。与模型组比较,青礞石高、中、低剂量组P38 MAPK磷酸化(p-P38 MAPK)表达显著降低(P<0.05),随着剂量的增加p-P38 MAPK表达逐渐减弱。结论:青礞石具有抗室性心律失常作用,抑制P38 MAPK信号通路是其作用途径之一。Objective:To observe the effects of Chloriti lapis on premature ventricular contractions(PVC),ventricular fibrillation(VF),and cardiac arrest(SCA)of rats induced by aconitine,to explore the mechanism.Methods:40 SD rats were randomly divided into model group and Chloriti lapis low,medium and high doses groups,and the rectal drug intake was intervened for 2 weeks.One hour after the last administration,The rats were anesthetized and fixed in the dorsal position.Intraperitoneal anesthesia with 20%urethane(1g·kg)was pumped into aconitine 20μg/kg through the femoral vein at a constant speed of 5μg/min.The incidence and onset time of PVC,VF,and SCA,aconitine poisoning dose were recorded.The expression of P38 mitogen activated protein kinase(MAPK)was detected by Western blot.Results:Compared with the model group,the incidence and onset time of PVC,VF,and SCA in the high,medium,and low dose groups of Chloriti lapis were significantly reduced(P<0.05).Compared with the model group,the consumption of aconitine that induced PVC,VF,and SCA of the high,medium,and low dose groups of Chloriti lapis were all increased(P<0.05).Compared with the model group,as the dosage of Chloriti lapis increased,the expression of phosphorylation of P38 MAPK(p-P38 MAPK)was significantly decreased(P<0.05).Conclusion:Chloriti lapis has anti-aconitine induced ventricular arrhythmias,inhibiting the P38 MAPK signaling pathway was one of the mechanism.

关 键 词：青礞石 乌头碱 室性心律失常 P38丝裂原激活蛋白激酶 大鼠 

分 类 号：R285.5[医药卫生—中药学]