白皮杉醇对卵巢癌细胞增殖、迁移、侵袭的影响及其诱导凋亡作用的实验研究  

Experimental Study on Effects of Piceatannol on Proliferation,Migration and Invasion of Ovarian Cancer Cells and Its Inducing Apoptosis Effect

作　　者：汪丽娜 任若瑜 何富乐 WANG Li’na;REN Ruoyu;HE Fule(Department of Pharmacy,Yongkang Hospital of Chinese Medicine,Yongkang,Zhejiang 321300;First Clinical Medical College,Zhejiang Chinese Medicine University,Hangzhou,Zhejiang 310053)

机构地区：[1]永康市中医院药剂科,浙江永康321300 [2]浙江中医药大学第一临床医学院,浙江杭州310053

出　　处：《中国中医药科技》2023年第5期866-870,共5页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省基础公益研究计划项目(LGF20H270010)。

摘　　要：目的:探究白皮杉醇对人卵巢癌SKOV-3细胞增殖、迁移和侵袭的影响以及相关的作用机制。方法:采用CCK8法检测不同浓度的白皮杉醇对SKOV-3细胞增殖能力的影响;采用细胞划痕实验检测不同浓度的白皮杉醇对SKOV-3细胞迁移能力的影响;采用Transwell侵袭实验检测不同浓度的白皮杉醇对SKOV-3细胞侵袭能力的影响;采用DAPI染色法检测不同浓度白皮杉醇对SKOV-3细胞凋亡的影响;采用Western blot实验检测SKOV-3细胞核增殖抗原(Ki67)、B淋巴细胞瘤-2(Bcl-2)、BCL-2相关X蛋白(Bax)和活化的半胱氨酸蛋白酶(Cleaved caspase-3)的表达水平。结果:白皮杉醇能够明显抑制SKOV-3细胞的增殖能力,明显降低SKOV-3细胞的迁移能力和侵袭能力;明显提高SKOV-3细胞的凋亡水平;白皮杉醇干预后,SKOV-3细胞Ki67和Bcl-2蛋白表达显著降低,Bax和Cleaved caspase-3蛋白表达显著升高。结论:白皮杉醇能够有效抑制SKOV-3细胞的增殖、迁移和侵袭,并能够诱导SKOV-3细胞凋亡。Objective:To investigate the effects of Piceatannol on proliferation,migration,and invasion of human ovarian cancer SKOV-3 cells and its inducing apoptosis and the related mechanisms.Methods:CCK8 assay was used to detect the effect of different concentrations of Picerlitol on the proliferation of SKOV-3 cells,cell scratch assay was used to detect the effect of different concentrations of Picerlitol on the migration ability of SKOV-3 cells,transwell invasion assay was used to detect the effect of different concentrations of Piceatannol on the invasion ability of SKOV-3 cells,DAPI staining was used to detect the effect of different concentrations of Picerlitol on apoptosis of SKOV-3 cell.The expression levels of Ki67,Bcl-2,Bax and Cleaved caspase-3 in SKOV-3 cells were detected by Western blot assay.Results:Piceatannol could significantly inhibit the proli-feration of SKOV-3 cells,inhibit the migration and invasion of SKOV-3 cells,increase the apoptosis of SKOV-3 cells.After Piceatannol intervention,the protein expressions of Ki67 and Bcl-2 in SKOV-3 cells were significantly decreased,while the protein expressions of Bax and Cleaved caspase3 were significantly increased.Conclusion:Piceatannol could effectively inhibit the proliferation,migration and invasion of SKOV-3 cells,and induce the apoptosis of SKOV-3 cells.

关 键 词：卵巢癌SKOV-3细胞 白皮杉醇 增殖 迁移 侵袭 凋亡 体外实验 

分 类 号：R285[医药卫生—中药学]