温压装药爆炸模拟气体对大鼠神经行为及海马神经发生的影响  

Effects of simulated gas of warm pressure charge explosion on neurobehavior and hippocampal neurogenesis in rats

作　　者：马宁 高俊宏 王鸿 卢青 范小琳 李亮 王琪 Ma Ning;Gao Junhong;Wang Hong;Lu Qing;Fan Xiaolin;Li Liang;Wang Qi(Institute for Hygiene of Ordnance Industry,Xi’an 710065,China)

机构地区：[1]兵器工业卫生研究所,西安710065

出　　处：《中华行为医学与脑科学杂志》2023年第8期680-687,共8页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国防基础加强计划基金项目(JCJQ2020-03)。

摘　　要：目的探讨温压装药爆炸模拟气体对大鼠神经行为及海马神经发生的影响。方法选取48只8~10周龄SPF级雄性SD大鼠,按照随机数字表法分为对照组、5 min暴露组、10 min暴露组和15 min暴露组,每组12只。吸入染毒后28 d,以高架十字迷宫评估大鼠焦虑样行为,双向主动回避实验评估学习、记忆功能;免疫荧光染色法检测海马齿状回神经干细胞(neural stem cells,NSCs)标志分子神经上皮细胞蛋白(SOX2)、成熟神经元标志分子神经元核抗原(neuronal nuclei,NeuN)的阳性表达情况;Western blot检测海马组织SOX2、NeuN蛋白表达量。采用GraphPad prism 8.0进行数据分析与制图,重复测量设计资料的比较采用重复测量方差分析,其他数据多组间比较采用单因素方差分析,两两比较采用Tukey法。利用Image J工具对海马神经细胞进行计数。结果(1)高架十字迷宫实验结果显示,4组大鼠进入开臂次数百分比、开臂停留时间百分比均差异有统计学意义(F=22.31,5.43,均P<0.05)。5 min、10 min、15 min暴露组大鼠进入开臂次数百分比[(28.85±1.47)%,(15.04±4.69)%,(12.66±2.89)%]、开臂停留时间百分比[(12.12±2.64)%,(12.16±1.11)%,(8.73±3.52)%]均低于对照组[(65.40±1.86)%,(42.92±3.12)%](均P<0.05),各暴露组两两比较均差异无统计学意义(均P>0.05)。(2)重复测量方差分析结果显示,主动回避获得期各组大鼠主动回避次数的分组与时间的交互作用、组别主效应与时间主效应均显著(F=4.21,16.64,56.46,均P<0.05)。4组大鼠第4天与第1天主动回避次数的差值差异有统计学意义(F=68.63,P<0.05)。主动回避记忆再现期结果显示,4组大鼠主动回避次数、主动回避反应时间均差异有统计学意义(F=8.17,8.28,均P<0.05)。10 min、15 min暴露组主动回避次数[(2.50±0.26)次,(2.33±0.06)次]低于对照组[(8.33±3.72)次](均P<0.05),主动回避反应时间[(6.25±0.40)s]、[(6.61±1.63)s]高于对照组[(3.69±1.41)s](均P<0.05)。1Objective To investigate the effects of thermobaric charge explosion simulated gas on long-term neurobehavior and hippocampal neurogenesis in rats.Methods A total of 48 male SPF grade SD rats aged 8-10 weeks were randomly divided into control group,5 min exposure group,10 min exposure group and 15 min exposure group,with 12 rats in each group.Twenty-eight days after inhalation of infection,the anxiety-like behavior of rats was evaluated by an elevated cross maze,and the learning and memory function of rats was evaluated by two-way active avoidance experiment.The number of positive cells of rat hippocampal dentate gyrus neural stem cells marker molecule neural epithelial cell protein(SOX2)and mature neuron marker molecular neuronal nuclei(NeuN)was detected by immunofluorescence staining.Western blot was used to detect SOX2 and NeuN protein expression in the hippocampal tissues of rats.GraphPad prism 8.0 software was used for data analysis.The comparison of repeated measurement design data was carried out by repeated measurement ANOVA.One-way ANOVA was used for inter group comparisons,and Tukey test was used for pairwise comparison.Hippocampal nerve cells were counted using the Image J software.Results(1)The experimental results of the elevated cross maze showed that the percentage of arm opening and the percentage of open arm residence time in each group had significant group effects(F=22.31,5.43,all P<0.05).The percentage of open arm entry times of rats in the 5 min,10 min and 15 min exposure group((28.85±1.47)%,(15.04±4.69)%,(12.66±2.89)%)and the percentage of residence time in open arm((12.12±2.64)%,(12.16±1.11)%,(8.73±3.52)%)were all lower than those of the control group((65.40±1.86)%,(42.92±3.12)%)(all P<0.05).There were no statistically significant differences in pairwise comparison among the three exposure groups(all P>0.05).(2)During the memory acquisition period,the results of repeated-ANOVA showed that the time main effect(F=56.46),the group main effect(F=16.64)and the interaction effect had sign

关 键 词：温压装药 行为学 神经干细胞 神经元 大鼠 

分 类 号：R82[医药卫生—临床医学]