A Preliminary Study of Cell Membrane Mediated Immobilization of a Recombinant Acyl-homoserine Lactonase AidH  

作　　者：蒋嘉峰 XIAO Lan LI Qichang 郭君慧 谢浩 JIANG Jiafeng;XIAO Lan;LI Qichang;GUO Junhui;XIE Hao(School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of Technology,Wuhan,430070,China)

机构地区：[1]School of Chemistry,Chemical Engineering and Life Sciences,Wuhan University of Technology,Wuhan,430070,China

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2023年第4期924-928,共5页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China(No.31771032)。

摘　　要：The aim of this work was to inhibit biofilm formation by taking advantages of bacterial surface display technology in combination with cell membrane chromatography.A recombinant protein INPAidH was constructed by fusing a quorum signal hydrolase AidH to the C-terminus of the ice nucleation protein(INP).Expression of INP-AidH was achieved on E.coli cell surface at an expression level of 30%of total membrane proteins.Activity of INP-AidH on cell membranes was confirmed in degrading the quorum signal C6-HSL as well as inhibiting bacterial biofilm.Immobilization of INP-AidH anchored cell membranes on silica gel particles was facilitated by taking advantages of cell membrane chromatography.The functionalized silica gel particles also exhibit activities in degrading C6-HSL and inhibiting bacterial biofilm.This article presents a new approach to prevent biofilm formation of silica-based materials.

关 键 词：quorum sensing N-acyl homoserine lactones(AHLs) INP-AidH BIOFILM IMMOBILIZATION 

分 类 号：Q814.2[生物学—生物工程]