电针“夹脊穴”对退变腰椎间盘模型兔背根神经节Netrin-1及其受体表达影响  

Effect of Electroacupuncture at"Jiaji"(EX-B2)on the Expression of Ganglion Conduction Protein 1 and Its Receptor in Dorsal Root Ganglion of Rabbits with Degenerative Lumbar Intervertebral Disc

作　　者：周婷 邹璟 黄国付 ZHOU Ting;ZOU Jing;HUANG Guo-fu(College of Acupuncture-Moxibustion and Orthopedics,Hubei University of Chinese Medicine,Wuhan,430065;Department of Acupuncture,Wuhan Hospital of Integrative Medicine,Wuhan,430030;Department of Chinese Medicine,Longgang District Maternity&Child Healthcare Hospital of Shenzhen City,Guangdong,518172)

机构地区：[1]湖北中医药大学针灸骨伤学院,武汉430065 [2]武汉市中西医结合医院针灸科,武汉430030 [3]深圳市龙岗区妇幼保健院中医科,广东518172

出　　处：《中国中西医结合杂志》2023年第8期963-968,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(No.82074517,No.81774392)。

摘　　要：目的观察电针“夹脊穴”对退变腰椎间盘模型兔椎间盘和神经根形态、神经轴突导向因子(Netrin-1)及其受体表达的影响。方法25只雄性新西兰大白兔按随机数字表法分为正常组、假模型组、模型组、模型+电针组和模型+假电针组,每组5只。除正常组外,其余各组以持续轴向加压L4椎间盘制备兔腰椎间盘退变模型,其中假模型组不予加压。造模成功后对模型+电针组电针L4、L5双侧“夹脊穴”;模型+假电针针刺后接入电针,不予电刺激;正常组、模型组和假模型组予以相同的捆绑,不予电针治疗,干预时间为20 min/次,1次/天,6次/周,共4周。造模术后评估各组大白兔的疼痛程度;治疗结束后,MRI观察各组大白兔椎间盘变化情况;Western Blot检测背根神经节(DRG)中Netrin-1及其受体的表达;电镜观察各组大白兔神经根的形态结构变化。结果干预后,与正常组比较,模型组大白兔一般情况不佳;疼痛程度总分及各项评分升高(P<0.01);造模节段椎间盘内髓核信号强度降低,椎间隙变窄;相应节段DRG中Netrin-1蛋白表达下降,其受体非协调性分子5同源蛋白B(UNC5B)蛋白表达下降,结肠癌缺失基因(DCC)表达升高(P<0.01);神经根组织结构破坏严重,神经纤维排列紊乱。与模型组比较,模型+电针组一般情况改善明显;疼痛程度总分及各项评分降低(P<0.05);造模节段椎间盘高度正常,髓核信号增强;DRG中Netrin-1蛋白表达水平升高,其受体UNC5B蛋白表达水平升高,DCC蛋白表达水平下降(P<0.01);神经根结构尚完整,损伤情况明显改善。与模型+电针组比较,模型+假电针组一般情况改善不明显;疼痛程度总分及各项评分升高(P<0.05);造模节段椎间盘内髓核信号较低;DRG中Netrin-1蛋白表达水平下降,其受体UNC5B蛋白表达水平下降,DCC蛋白表达水平升高(P<0.01);神经根结构破坏。结论电针可调节DRG中Netrin-1蛋白及其相关受体的表达,抑制DRG神Objective To observe the effects of electroacupuncture at Jiaji(EX-B2)on the morphology of intervertebral disc and nerve root,and the expressions of neurodirecting factor 1(Netrin-1)and its receptor in rabbits with degenerative lumbar intervertebral disc.Methods Twenty-five male New Zealand rabbits were divided into normal group,sham-model group,model group,model+electroacupuncture group and model+sham-electroacupuncture group according to random digit table,5 rats in each group.Except normal group,the rabbit model of lumbar intervertebral disc degeneration was prepared by continuous axial compression of L4 intervertebral disc in the rest 4 groups.No pressure was given to rabbits in sham-model group.After successful modeling,rabbits in the model+electroacupuncture group were intervened with electroacupuncture,and bilateral L4 and L5 Jiaji(EX-B2)were selected,once a day for 20 min.Rabbits in the model+sham electroacupuncture were connected with electroacupuncture,but with no electroacupuncture stimulation.The same binding was performed to rabbits in normal,model,and sham model groups with no electroacupuncture.Six days consisted of a course of treatment for a total of 4 treatment courses.After modeling the pain degree of rabbits in each group was evaluated.After treatment the changes of intervertebral disc in each group were observed by MRI.The expressions of Netrin-1 and its receptor in dorsal root ganglion(DRG)were detected by Western Blot.And the morphological and structural changes of nerve roots in each group were observed under electron microscope.Results After the intervention,as compared with the normal group,the general condition of rabbits in the model group was not so good.The total pain score and various scores increased(P<0.01).The signal intensity of the nucleus pulposus in the modeled segmental intervertebral disc decreased and the intervertebral space narrowed.The Netrin-1 protein expression in the DRG of corresponding intervertebral disc decreased,its expression of the receptor UNC5B protein decre

关 键 词：电针 兔 椎间盘退变 背根神经节 神经轴突导向因子-1 结肠癌缺失基因 非协调性分子5同源蛋白B 

分 类 号：R245.97[医药卫生—针灸推拿学]