约氏疟原虫P.y 17XL感染小鼠长链非编码RNA表达谱特征分析  

Characteristics of long non-coding RNA expression profiles in mice infected with P.y 17XL

作　　者：吕荫宜 吴双 田爽 廖国艳 杜云婷 陈光[1,2] LYU Yin-yi;WU Shuang;TIAN Shuang;LIAO Guo-yan;DU Yun-ting;CHEN Guang(College of Basic Medicine,Jiamusi University,Jiamusi 154000,China;Taizhou College,Taizhou 318000,China;Hebei North University,Zhangjiakou 075000,China;Department of Clinical Laboratory,Tumor Hospital of China Medical University,Liaoning Tumor Hospital,Shenyang 110042,China)

机构地区：[1]佳木斯大学基础医学院,佳木斯154000 [2]台州学院医学院,台州318000 [3]河北北方学院,张家口075000 [4]中国医科大学肿瘤医院,辽宁省肿瘤医院检验科,沈阳110042

出　　处：《中国人兽共患病学报》2023年第8期733-738,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.81101278);台州学院杰出青年项目(No.2019JQ003)。

摘　　要：目的探究长链非编码RNA(lncRNA)在疟原虫感染过程中表达谱变化特征。方法建立P.y 17XL感染BALB/c鼠疟模型,计数红细胞感染率,记录生存率;使用RNA-seq方法确认P.y 17XL感染的BALB/c小鼠lncRNA的差异表达谱。GO和KEGG分析以阐明lncRNAs在疟疾感染过程中对免疫功能的调节作用。结果与正常对照组小鼠相比,P.y 17XL感染BALB/c小鼠脾脏中lncRNAs表达谱发生明显改变,132个lncRNAs表达上调,159个lncRNAs表达下调。通过GO和KEGG分析发现,明显差异表达的lncRNAs包括3个已知和9个新发现的优势lncRNAs,即ENSMUST00000146154.1、ENSMUST00000181191.1、ENSMUST00000202081.1、LNC_000258、LNC_000871、LNC_000962、LNC_002093、LNC_004354、LNC_006284、LNC_004518、LNC_004350和LNC_003730均与疟疾免疫应答密切相关。结论P.y 17XL感染改变了宿主lncRNAs的表达谱,宿主-寄生虫相互作用通过lncRNAs调控了抗疟疾免疫应答。本研究为进一步研究疟原虫与宿主细胞之间作用机制提供理论依据。This study explored the characteristics of long non-coding RNA(lncRNA)expression profiles in the process of malaria infection.BALB/c mice were infected with P.y 17XL,and parasitemia and survival rates were monitored.RNA-seq was used to confirm the differential expression of lncRNAs in P.y 17XL-infected BALB/c mice.GO and KEGG analysis were performed to elucidate the regulatory roles of lncRNAs in immune function in Malaria.Analysis of significant changes in lncRNA expression revealed 132 up-regulated and 159 down-regulated lncRNAs.GO and KEGG analysis indicated that the clearly differentially expressed lncRNA included three known and nine novel lncRNAs(ENSMUST00000146154.1,ENSMUST00000181191.1,ENSMUST00000202081.1,LNC_000258,LNC_000871,LNC_000962,LNC_002093,LNC_004354,LNC_006284,LNC_004518,LNC_004350 and LNC_003730)that correlated with the immune response in malaria.P.y 17XL infection altered the host lncRNA expression profile.Host-parasite interaction regulates anti-malaria immunity via lncRNAs.This study is expected to provide a theoretical basis for further research on the interaction mechanism between Plasmodium and host cells.

关 键 词：疟原虫 长链非编码RNA 免疫信号 免疫应答 

分 类 号：R382.3[医药卫生—医学寄生虫学]