一株硝基还原假单胞菌的溶磷特性及对碳循环相关基因的影响  

Phosphorus solubilizing characteristics of a Pseudomonas nitroreducens strain and its effect on carbon cycling related genes

作　　者：乔志伟 刘超 王红 QIAO Zhi-wei;LIU Chao;WANG Hong(College of Agriculture,Anshun University,Anshun 561000,China;College of Resources and Environmental Engineering,Anshun University,Anshun 561000,China;Rural Revitalization Research Center of Guizhou Universities,Anshun 561000,China)

机构地区：[1]安顺学院农学院,贵州安顺561000 [2]安顺学院资源与环境工程学院,贵州安顺561000 [3]贵州省高校乡村振兴研究中心,贵州安顺561000

出　　处：《江苏农业学报》2023年第5期1151-1158,共8页Jiangsu Journal of Agricultural Sciences

基　　金：贵州省科技计划项目(黔科合基础20181401);贵州省普通高等学校科技拔尖人才支持计划项目(黔教合KY字2017091);贵州省教育厅青年科技人才成长项目(黔教合KY字2017289)。

摘　　要：为提高黔中黄壤区农田土壤磷素的有效性,以从该地区筛选的1株具有溶磷能力的硝基还原假单胞菌(QHR-9)为试验菌株,通过室内摇瓶培养试验研究菌株的溶磷特性。设置对照、QHR-9、QHR-9+葡萄糖、QHR-9+组合糖类等4个处理,通过室外土壤培养试验研究菌株在土壤中的作用及对碳循环相关基因的影响。室内摇瓶培养试验结果表明,培养第7 d,QHR-9对磷酸三钙、磷酸铝、磷矿粉的溶解能力分别为645.82 mg/L、269.17 mg/L、272.45 mg/L;QHR-9在10种不同碳源条件下溶磷能力为28.06~645.82 mg/L,在10种组合碳源条件下的溶磷能力为92.31 mg/L。室外土壤培养试验结果表明,QHR-9可以显著增加土壤有效磷含量、酸性磷酸酶和碱性磷酸酶活性、硝基还原假单胞菌丰度等,QHR-9+葡萄糖与QHR-9+组合糖类处理上述4项指标均高于QHR-9处理;土壤pH以QHR-9+葡萄糖处理最低,与QHR-9、QHR-9+组合糖类处理差异不显著;QHR-9处理微生物碳循环糖基转移酶(GT)和辅助活性酶(AA)基因相对丰度与对照相比降低,糖苷水解酶(GH)基因相对丰度增加,QHR-9+葡萄糖和QHR-9+组合糖类处理加剧了这一变化趋势。相关性分析结果表明,土壤有效磷含量、pH、酸性(碱性)磷酸酶活性与微生物碳循环相关基因相对丰度均有显著或极显著相关性。To improve soil phosphorus availability in yellow soil area farmland of central Guizhou,a Pseudomonas nitroreducens strain(QHR-9)with phosphorus-solubilizing ability was selected from this area as the test strain.The phosphorus solubilization characteristics of the strain were studied by laboratory shaking flask culture test.Outdoor soil culture experiments were conducted to study the effect of the strain in soil and its effect on carbon cycling related genes,based on four treatments which contained CK,QHR-9,QHR-9+glucose and QHR-9+combined carbohydrate.Results of laboratory shaking flask culture test showed that,the dissolution capacity of QHR-9 to tricalcium phosphate,aluminum phosphate and ground phosphorite were 645.82 mg/L,269.17 mg/L and 272.45 mg/L respectively on the 7 th day of culturing.The phosphorus solubilizing capacity of QHR-9 was 28.06-645.82 mg/L under ten carbon sources conditions,the phosphorus solubilizing capacity under the combined ten carbon source condition was 92.31 mg/L.Results of outdoor soil culture experiments showed that,QHR-9 could significantly increase soil available phosphorus content,acid phosphatase activity,alkaline phosphatase activity and relative abundance of Pseudomonas nitroreducens strain,etc.Under QHR-9+glucose treatment and QHR-9+combined carbohydrate treatment,the above four indexes were significantly higher than those under QHR-9 treatment.Under QHR-9+glucose treatment,the pH was the lowest,and there was no significant difference between QHR-9 or QHR-9+combined carbohydrate treatment.Compared with CK,the relative abundance of glycosyltransferases(GT)and auxiliary actives(AA)genes of microbial carbon cycle under QHR-9 treatment decreased,while the relative abundance of glycoside hydrolases(GH)genes increased,the variation trend was exacerbated by QHR-9+glucose treatment and QHR-9+combined carbohydrate treatment.Correlation analysis showed that,soil available phosphorus content,pH,acid phosphatase or alkaline phosphatase activity were significantly or extremely signifi

关 键 词：硝基还原假单胞菌 溶磷特性 不同碳源 碳循环基因 

分 类 号：S154.3[农业科学—土壤学]