1株红树来源真菌Penicillium sp.次级代谢产物及其生物活性研究  

Secondary metabolites of the mangrove-derived fungus Penicillium sp.and their biological activities

作　　者：胡洁 武艳伟 王翠芳[1] 翟瑞芹 邵长伦[1] 郑纪勇[2] 魏美燕[1] HU Jie;WU Yanwei;WANG Cuifang;ZHAI Ruiqin;SHAO Changlun;ZHENG Jiyong;WEI Meiyan(Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China,Qingdao 266003,China;State Key Laboratory for Marine Corrosion and Protection,Luoyang Ship Material Research Institute(LSMRI),Qingdao 266237,China)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003 [2]中国船舶重工集团公司第七二五研究所海洋腐蚀与防护重点实验室,山东青岛266237

出　　处：《中国海洋药物》2023年第4期13-18,共6页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目(U1706210,41322037)资助。

摘　　要：目的研究1株红树来源真菌Penicillium sp.中的次级代谢产物及生物活性。方法综合运用硅胶柱层析(CC)和半制备型高效液相色谱(HPLC)等方法分离纯化次级代谢产物,利用核磁共振(NMR)及质谱(MS)等现代波谱分析方法鉴定化合物结构,以斑马鱼血管抑制模型对其进行活性评价。结果在细胞毒活性的指导下,从Penicillium sp.中分离出抗肿瘤活性化合物brefeldin A,同时还分离出5个天然产物,分别是(+)-semivioxanthin(1)、(-)-st r iatisporolideA(2)、dibut ylphthalate(3)、bisdethiobis(methylthio)-acetylapoaranotin(4)和alternarosin A(5)。其中化合物1和3分别在1和10μg/mL浓度下显示出斑马鱼血管抑制活性,但同时会导致一定的脊柱弯曲。化合物3还具有抑制硅藻附着的潜在防污活性。结论化合物4和5为首次从真菌Penicillium sp.中分离获得。化合物1和3具有潜在的生物活性。Objective To study the secondary metabolites from the mangrove-derived fungus Penicillium sp.and evaluate their biological activities.Methods The compounds were separated and purified by silica gel column chromatography(CC) and semi-preparative high performance liquid chromatography(HPLC).Their structures were identified by modern spectroscopic analysis including nuclear magnetic resonance(NMR) and mass spectrometry(MS) methods.All the compounds were tested for zebrafish anti-angiogenic and antimicroalgae activities.Results Under the guidance of cytotoxic activity,brefeldin A,an anti-tumor activity natural compound,and five compounds including(+)-semivioxanthin(1),(-)-striatisporolide A(2),dibutyl phthalate(3),bisdethiobis(methylthio)-acetylapoaranotin(4) and alternarosin A(5) were obtained from the fungus Penicillium sp..Compounds 1 and 3 exhibited anti-angiogenic activities to zebrafish embryo at the concentration of 1 and 10 μg/mL,associated with notochord malformation and tail malformation(28 h).Compound 3 showed potential antifouling activity against Navicula leavissima.Conclusion Compounds 4 and 5 were isolated from fungus Penicillium sp.for the first time and compounds 1 and 3 showed potential biological activities.

关 键 词：青霉属 次级代谢产物 斑马鱼血管抑制活性 

分 类 号：R931.77[医药卫生—生药学]