大高良姜、红豆蔻乙酸乙酯部位多组分含量测定  

Determination of Multi-component Content in Ethyl Acetate Parts of Dagaoliangjiang(Alpiniae Officinarum Rhizoma)and Hongdoukou(Galangae Fructus)

作　　者：黄少敏 丘海冰 谢旭格 牛新迈 陈智兰 赵天琪 覃金桥 秦华珍[1] HUANG Shaomin;QIU Haibing;XIE Xuge;NIU Xinmai;CHEN Zhilan;ZHAO Tianqi;QIN Jinqiao;QIN Huazhen(Guangxi University of Traditional Chinese Medicine,Nanning 530200,China;Yulin Normal University,Yulin 537000,China)

机构地区：[1]广西中医药大学,广西南宁530200 [2]玉林师范学院,广西玉林537000

出　　处：《山东中医药大学学报》2023年第5期637-646,共10页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:82074034);广西中医药大学硕士研究生教育创新计划项目(编号:YCXJ2021019)。

摘　　要：目的:以同基原不同药用部位的中药大高良姜与红豆蔻为研究对象,建立多组分含量测定方法。方法:以不同产地的大高良姜、红豆蔻乙酸乙酯部位为样品,运用高效液相色谱(HPLC)法进行测定。采用Inertsil ODS-3(4.6 mm×250 mm,5μm)色谱柱;流动相:乙腈(A)-0.05%冰乙酸水溶液系统(B),流速0.8 mL·min-1;柱温30℃;检测波长:270 nm;进样量:10μL。对方法进行专属性、标准曲线的建立、精密度、稳定性、重复性、加样回收考察。对其指认的共有成分进行含量测定。结果:测定方法稳定可靠,空白溶剂无干扰以及混合对照品溶液专属性高,仪器精密度和方法重复性良好,加样回收率达到含量测定要求。测得10个不同产地的大高良姜乙酸乙酯部位指认的多组分原儿茶酸、原儿茶醛、对羟基苯甲酸、香草酸、芦丁、对羟基苯甲醛含量分别为0.4698~1.3998 mg·g^(-1)、0.1287~0.2953 mg·g^(-1)、0.6280~1.8224 mg·g^(-1)、0.5825~1.1339 mg·g^(-1)、0.9641~2.0190 mg·g^(-1)、3.9424~7.9024 mg·g^(-1);红豆蔻乙酸乙酯部位指认的多组分原儿茶酸、原儿茶醛、对羟基苯甲酸、香草酸、芦丁、对羟基苯甲醛含量分别为0.8376~2.0446 mg·g^(-1)、0.3054~0.5845 mg·g^(-1)、0.9259~1.6094 mg·g^(-1)、0.3474~0.6445 mg·g^(-1)、4.8377~26.7308 mg·g^(-1)、2.6081~3.9132 mg·g^(-1)。结论:建立了HPLC同时检测大高良姜、红豆蔻乙酸乙酯部位中原儿茶酸、原儿茶醛、对羟基苯甲酸、香草酸、芦丁、对羟基苯甲醛等6个组分含量的测定方法,该方法准确、可靠。Objective:To establish a multi-component content determination method through taking different medicinal parts of the same origin of Dagaoliangjiang(Alpiniae Officinarum Rhizoma)and Hongdoukou(Galangae Fructus)as the object of study.Methods:The ethyl acetate parts of Dagaoliangjiang and Hongdoukou from different producing areas were used as samples for determination by high performance liquid chromatography(HPLC).The chromatographic column was Inertsil ODS-3(4.6 mm×250 mm,5μm).Mobile phase:acetonitrile(A)-0.05%glacial acetic acid aqueous solution system(B).Flow rate was 0.8 mL·min-1 and the column temperature was 30℃.Detection wavelength:270 nm.Injection volume:10μL.The specificity,establishment of standard curve,precision,stability,repeatability and sampling recovery of the method were investigated.The contents of the identified common components were determined.Results:The determination method of this study is stable and reliable,the blank solvent has no interference and the mixed reference solution has high specificity,the instrument precision and the method repeatability are good,and the sample recovery rate meets the content determination requirements.The contents of protocatechuic acid,protocatechuic aldehyde,p-hydroxybenzoic acid,vanillic acid,rutin and p-hydroxybenzaldehyde identified in the ethyl acetate parts of Dagaoliangjiang from 10 different producing areas were 0.4698~1.3998 mg·g^(-1),0.1287~0.2953 mg·g^(-1),0.6280~1.8224 mg·g^(-1),0.5825~1.1339 mg·g^(-1),0.9641~2.0190 mg·g^(-1),3.9424~7.9024 mg·g^(-1)respectively.The contents of protocatechuic acid,protocatechuic aldehyde,p-hydroxybenzoic acid,vanillic acid,rutin and p-hydroxybenzaldehyde identified in the ethyl acetate parts of Hongdoukou were 0.8376~2.0446 mg·g^(-1),0.3054~0.5845 mg·g^(-1),0.9259~1.6094 mg·g^(-1),0.3474~0.6445 mg·g^(-1),4.8377~26.7308 mg·g^(-1),2.6081~3.9132 mg·g^(-1)respectively.Conclusions:A HPLC method has been established for simultaneous determination of six components such as protocatechuic acid,protocate

关 键 词：大高良姜 红豆蔻 乙酸乙酯部位 精密度 稳定性 重复性 加样回收 多组分含量 

分 类 号：R284[医药卫生—中药学] R917[医药卫生—中医学]