栀子中环烯醚萜类成分与黄色素类成分减肥作用比较研究  

Comparative Study on Weight Loss Effects of Iridoids and Yellow Pigments in Zhizi(Gardeniae Fructus)

作　　者：杜洁 党心语 王民娴 林桂涛[1] 梁海月 DU Jie;DANG Xinyu;WANG Minxian;LIN Guitao;LIANG Haiyue(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Yantai Food and Drug Inspection and Testing Center,Yantai 264003,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]烟台市食品药品检验检测中心,山东烟台264003

出　　处：《山东中医药大学学报》2023年第5期656-661,共6页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省重点研发计划(重大关键技术及重点产业关键技术)项目(编号:2016CYJS08A01-10)。

摘　　要：目的:比较栀子中环烯醚萜类成分与黄色素类成分对大鼠减肥作用的差异,分析栀子发挥减肥作用的有效成分。方法:将40只雄性SD大鼠随机分为对照组及栀子环烯醚萜组、黄色素组和75%乙醇提取物组,各组分别给予生理盐水、栀子环烯醚萜类成分、栀子黄色素类成分和栀子75%乙醇提取物灌胃,连续灌胃30 d;前10 d以普通饲料喂养,后20 d以高脂饲料喂养。检测各组大鼠体质量增长率、粪便总脂、脏器指数,测定血清高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、甘油三酯(TG)、胆囊收缩素(CCK)、胰岛素(INS)、胃泌素(GAS)、胰高血糖素-1(GLP-1)含量。结果:与对照组比较,在开始10 d的普通饲料喂养阶段,各给药组大鼠体质量增长率均降低(P<0.05或P<0.01);高脂饮食阶段,即第11至20天、第21至30天,栀子环烯醚萜组和75%乙醇提取物组大鼠体质量增长率降低(P<0.05或P<0.01),且比两组大鼠总体体质量增长率显著降低(P<0.01)。与对照组比较,栀子黄色素组、75%乙醇提取物组大鼠粪便总脂含量增加(P<0.05);栀子环烯醚萜组和75%乙醇提取物组大鼠附睾脂肪率降低(P<0.05)、HDL-C含量升高(P<0.01)、LDL-C及TG含量均降低(P<0.01或P<0.05)、CCK含量升高(P<0.05或P<0.01);栀子环烯醚萜组、黄色素组、75%乙醇提取物组INS含量降低(P<0.05或P<0.01);各给药组大鼠GAS含量比较,差异无统计学意义(P>0.05);栀子75%乙醇提取物组GLP-1含量高于对照组(P<0.01)。结论:栀子抑制食欲的主要成分为环烯醚萜类成分,其通过增加CCK的分泌发挥作用;栀子黄色素类成分对普通饮食有一定抑制作用,可减少脂肪吸收,发挥一定的减肥作用;而栀子75%乙醇提取物是两者作用之和,其抑制体质量增长作用最佳。To compare the effects of iridoids and yellow pigments of Zhizi(Gardeniae Fructus)on weight loss in rats,and to analyze the effective components of Zhizi for weight loss.Methods:Total 40 male SD rats were randomly divided into the control group,and Zhizi iridoid group,yellow pigment group,75%ethanol extract group.Each group was given the normal saline,iridoids of Zhizi,Zhizi yellow pigments and 75%ethanol extract of Zhizi by gavage for 30 days.The first 10 days were fed with regular feed,and the last 20 days were fed with high-fat feed.The growth rate of body mass,total fecal fat and organ index of rats in each group were measured,and the contents of serum high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),triglyceride(TG),cholecystokinin(CCK),insulin(INS),gastrin(GAS)and glucagon-1(GLP-1)were determined.Results:Compared with the control group,the growth rate of body mass of rats in all drug administration groups decreased(P<0.05 or P<0.01)at the beginning of 10 days of normal feed.In the stage of high fat diet,i.e.,days 11 to 20 and 21 to 30,the growth rate of body mass in the Zhizi iridoid group and 75%ethanol extract group decreased(P<0.05 or P<0.01),and the overall growth rate of body mass in these two groups decreased significantly(P<0.01).Compared with the control group,the total fat content in feces of rats in Zhizi yellow pigment group and 75%ethanol extract group increased(P<0.05);The fat percentage of epididymis in the Zhizi iridoid group and 75%ethanol extract group decreased(P<0.05);The contents of HDL-C in the Zhizi iridoid group and 75%ethanol extract group increased(P<0.01),while the contents of LDL-C and TG decreased(P<0.01 or P<0.05);The contents of CCK in the Zhizi iridoid group and 75%ethanol extract group increased(P<0.05 or P<0.01);The contents of INS in the Zhizi iridoid group,yellow pigment group and 75%ethanol extract group decreased(P<0.05 or P<0.01);There was no significant difference in GAS content among all groups(P>0.05);GLP-1 content in the Zhizi 7

关 键 词：栀子环烯醚萜 栀子黄色素 抑制食欲 胆囊收缩素 胰岛素 甘油三酯 胰高血糖素-1 

分 类 号：R283[医药卫生—中药学]