苏云金芽孢杆菌BT蛋白动物安全性试验  

The Animal Safety Test of Bacillus thuringiensis' BT Protein

作　　者：刘鼎阔 董惠峰 宁未萌 刘阔 刘芳 李源 胡鹏程 LIU Dingkuo;DONG Huifeng;NING Weimeng;LIU Kuo;LIU Fang;LI Yuan;HU Pengcheng(Dingzheng Xinxing Biotechnology(Tianjin)Co.,Ltd.,Tianjin 300383,China;Tianjin Agricultural College,Tianjin 300393,China;Tianjin Key Laboratory of Biological Feed Additives,Tianjin 300383,China;Tianjin Feed Small Molecule Protein Engineering Center,Tianjin 300383,China)

机构地区：[1]鼎正新兴生物技术(天津)有限公司,天津300383 [2]天津农学院,天津300393 [3]天津市生物饲料添加剂企业重点实验室,天津300383 [4]天津市饲用小分子蛋白工程中心,天津300383

出　　处：《生物化工》2023年第4期39-41,45,共4页Biological Chemical Engineering

摘　　要：目的:明确高剂量条件下苏云金芽孢杆菌发酵液中BT蛋白对哺乳动物的生物安全性。方法:健康小鼠随机分为4组,每组10只;实验组分别按0.2 mL/kg、0.6 mL/kg、1.0 mL/kg灌服Bt发酵上清液,每天一次,连续给药7天;空白对照组正常饲养,不灌胃。结果:实验组与对照组血常规、血液生化分析等检测数据差异不显著;灌胃后解剖小鼠,无明显可见的病理变化;取心、肝、脾、肺、肾做组织切片,通过显微镜观测仍未见病理变化。结论:小鼠能够耐受高剂量苏云金芽孢杆菌发酵液中的BT蛋白。Objective:In order to clarify the biological safety of BT protein in Bacillus thuringiensis fermentation broth for mammals at high doses.Methods:The experiment is designed as follows:Healthy mice are randomly divided into 4 groups of 10 mice in each group.The experimental group is administered Bacillus thuringiensis fermentation supernatant once a day at 0.2 mL/kg,0.6 mL/kg and 1.0 mL/kg for 7 days.The blank control group is fed normally and don't gavage.Results:The test results show that blood routine and blood biochemical analysis are not significantly different between the experimental group and the control group;After gastric gavage dissecting the mice,there are no obvious pathological changes;The heart,liver,spleen,lungs,and kidneys are taken as tissue sections,and no pathological changes are seen by microscopic observation.Conclusion:This shows that the mice are able to tolerate the BT protein in the fermentation broth of Bacillus thuringiensis at high doses.

关 键 词：苏云金芽孢杆菌 BT蛋白 生物安全性 

分 类 号：S482.7[农业科学—农药学] R595.4[农业科学—植物保护]