基于脂质代谢组学研究矽肺大鼠模型中血浆脂质特征  

The characteristics of plasma lipids in silicosis rat models were studied based on lipid metabolomics

作　　者：何海兰 戚荣 崔洁 王宏丽 郝小惠 刘和亮 He Hailan;Qi Rong;Cui Jie;Wang Hongli;Hao Xiaohui;Liu Heliang(School of Public Health,North China University of Science and Technology,Tangshan 063210,China;Clinical Medical School,North China University of Science and Technology,Tangshan 063210,China;Hebei Key Laboratory of Organ Fibrosis,North China University of Science and Technology,Tangshan 063210,China)

机构地区：[1]华北理工大学公共卫生学院,唐山063210 [2]华北理工大学临床医学院,唐山063210 [3]河北省器官纤维化重点实验室,唐山063210

出　　处：《中华劳动卫生职业病杂志》2023年第8期569-575,共7页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金(81673119);河北省自然科学基金(H2022209021);河北省自然科学基金精准医学联合基金重点项目(H2022209039);河北省省级研究生创新资助项目(CXZZBS2022105)。

摘　　要：目的通过分析矽肺大鼠血浆脂质组学,筛选矽肺模型中的差异代谢物和差异代谢途径。方法于2021年5月,将20只雄性SD大鼠随机分为对照(C)组、矽肺1周(S1W)组、矽肺2周(S2W)组和矽肺4周(S4W)组,每组5只。通过气管灌注1 ml二氧化硅(SiO2)50 mg/ml混悬液或生理盐水构建大鼠矽肺模型,分别于1、2和4周处死大鼠,HE染色观察大鼠肺组织病理情况,利用高效液相色谱-离子淌度-四极杆飞行时间质谱仪(UPLC-IMS-QTOF)对血浆样本进行脂质组学分析,筛选出矽肺模型中潜在的差异代谢物并进行脂质富集分析。结果与对照组比较,S1W组大鼠肺组织有结节的形成,S2W组和S4W组中结节逐渐增大,肺泡结构逐渐被破坏。代谢组学筛选出S1W、S2W、S4W组中分别有14、24、28个差异代谢物,差异代谢物主要富集在甘油磷脂代谢,脂肪酸降解,糖基磷脂酰肌醇(GPI)的生物合成和脂肪酸伸长等代谢途径。结论矽肺大鼠模型中血浆脂质代谢物存在明显变化。Objective To screen the differential metabolites and metabolic pathways in silicosis model by analyzing plasma metabolomics of silicosis rats.Methods In May 2021,twenty male SD rats were randomly divided into control group(C),1-week silicosis group(S1W),2-week silicosis group(S2W)and 4-week silicosis group(S4W),with 5 rats in each group.Rats were intratracheally instillated with 1ml crystalline SiO2 suspension(50 mg/ml)or normal saline and were sacrificed after 1 week,2 weeks and 4 weeks,HE staining was used to observe the lung pathology of rats.The plasma samples were analyzed by UPLC-IMS-QTOF mass spectrometer to screen out potential differential metabolites in silicosis models and analyze their lipid enrichment.Results HE results showed that nodules formed in the silicosis model group,and with the extension of time,nodules gradually increased and alveolar structure was gradually destroyed.Metabolomics screened out 14 differential metabolites in S1W,24 in S2W,and 28 in S4W,and found that the differential metabolites were mainly enriched in the metabolism of glycerophospholipid metabolism,fatty acid degradation,Glycosylphosphatidylinositol(GPI)-anchor biosynthesis,fatty acid elongation and other metabolic pathways.Conclusion There are significant changes in plasma lipid metabolites in silicosis rat models.

关 键 词：矽肺 血浆 脂质代谢组学 

分 类 号：R135.2[医药卫生—劳动卫生]