CoxA16感染儿童手足口病患者IL-6、IFN-α水平变化及其与TLR9基因多态性的相关性  

Changes of IL-6 and IFN-αlevels in children with hand,foot and mouth disease infected by CoxA16 and their correlation with TLR9 gene polymorphism

作　　者：罗筱 白宝鑫 王爽[1] LUO Xiao;BAI Baoxin;WANG Shuang(Department of Clinical Laboratory,Shiyan Maternity and Child Health Hospital,Shiyan,Hubei 442000,China;Hubei Medical College,Shiyan,Hubei 442000,China)

机构地区：[1]十堰市妇幼保健院检验科,湖北十堰442000 [2]湖北医药学院,湖北十堰442000

出　　处：《重庆医学》2023年第17期2597-2601,2605,共6页Chongqing medicine

基　　金：湖北省科技项目(2019CFB326);湖北省十堰市引导性科研项目(21Y05)。

摘　　要：目的探讨柯萨奇病毒A16型(CoxA16)感染的儿童手足口病患者外周血白细胞介素-6(IL-6)、α干扰素(IFN-α)水平变化及其与Toll样受体9(TLR9)基因多态性间的关系。方法选取2019年4月至2021年10月十堰市妇幼保健院收治的201例CoxA16感染的手足口病患儿作为研究对象,根据是否出现中枢神经系统并发症分为轻症组(无中枢神经系统并发症,158例)和重症组(有中枢神经系统并发症,43例),选取同期体检的132例健康儿童作为对照组。采集各组外周血检测IL-6、IFN-α、TLR9表达水平,采用聚合酶链式反应-限制性内切酶片段长度多态性(PCR-RELP)技术检测TLR9基因(rs352140、rs164640、rs187084)位点多态性,分析以上基因多态性与儿童手足口病的相关性。结果轻症组、重症组IL-6、IFN-α、TLR9表达水平高于对照组,且重症组高于轻症组,差异有统计学意义(P<0.05)。TLR9基因(rs352140、rs164640、rs187084)位点多态性的预期基因型频率均处于Hardy-Weinberg平衡(P>0.05)。3组TLR9基因rs352140、rs164640位点基因型分布及等位基因分布比较,差异无统计学意义(P>0.05);而rs187084基因位点基因型及T、C等位基因分布比较,差异有统计学意义(P<0.05)。二元logistic回归分析表明,TLR9基因rs187084位点CC/TC基因型、C等位基因是儿童手足口病CoxA16感染的危险因素(P<0.05)。结论TLR9基因rs187084位点基因多态性与CoxA16型手足口病存在较大关联,并可能是CoxA16感染由轻症进展成重症的关键因子。Objective To investigate the changes of interleukin-6(IL-6)and interferon-α(IFN-α)levels in peripheral blood of children with hand,foot and mouth disease(HFMD)infected with coxsackievirus A16(CoxA16)and their relationship with toll-like receptor 9(TLR9)gene polymorphism.Methods A total of 201 children with HFMD infected with CoxA16 treated in Shiyan Maternal and Child Health Hospital from April 2019 to October 2021 were selected as the study objects,and according to whether there were central nervous system complications,the patients were divided into the mild group(158 cases without central nervous system complications)and the severe group(43 cases with central nervous system complications),and 132 healthy children who underwent physical examination during the same period were selected as the control group.The expression levels of IL-6,IFN-αand TLR9 protein were detected in peripheral blood of each group.The single nucleotide polymorphisms(SNP)of TLR9 gene(rs352140,rs164640,rs187084)were detected by polymerase chain react-restriction enzyme fragment length polymorphism(PCR-RELP),and the correlation between these gene polymorphisms and HFMD in children was analyzed.Results The protein expression levels of IL-6,IFN-αand TLR9 in the mild group and the severe group were higher than those in the control group,and the severe group was higher than that in the mild group,the differences were statistically significant(P<0.05).The expected genotype frequencies of SNP at TLR9 gene(rs352140,rs164640,rs187084)were in Hardy-Weinberg equilibrium(P>0.05).There were no significant differences in the genotype distribution and allele distribution of TLR9 gene rs352140 and rs164640 between the experimental group and the control group(P>0.05),but there were significant differences in the genotype distribution and T and C allele distribution of rs187084 gene locus(P<0.05).Further binary Logistic regression analysis showed that CC/TC genotype and C allele of TLR9 rs187084 were independent risk factors for CoxA16 infection in child

关 键 词：柯萨奇病毒A16 手足口病 白细胞介素-6 α干扰素 TOLL样受体9 基因多态性 

分 类 号：R725.1[医药卫生—儿科]