益脑康胶囊对缺血再灌注模型小鼠自噬相关因子Beclin-1、LC3B和p62的影响  

Effects of Yinaokang Capsules on Autophagy-related Factors Beclin-1,LC3B and p62 in Mice Model of Ischemia Reperfusion

作　　者：邓敏贞 钟晓琴[2] 高志杰 彭丽霖 程骁 孙景波 DENG Minzhen;ZHONG Xiaoqin;GAO Zhijie;PENG Lilin;CHENG Xiao;SUN Jingbo(Guangdong Provincial Hospital of Chinese Medicine/The Second Affiliated Hospital of Guangzhou University of Chinese Medicine/Guangdong Academy of Traditional Chinese Medicine,Guangzhou 510120,China;Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广东省中医院/广州中医药大学第二附属医院/广东省中医药科学院,广东广州510120 [2]广州中医药大学,广东广州510006

出　　处：《西部中医药》2023年第9期10-14,共5页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81904104);广东省中医药局科研基金(20211203);广州中医药大学2017年高水平大学建设计划(A1-AFD018171Z11096);广东省中医院中医药科学技术研究专项(YN2018ZD07)。

摘　　要：目的:研究益脑康胶囊对缺血再灌注模型小鼠自噬相关因子肌球蛋白样BCL2结合蛋白(myosin-like BCL2 interacting protein,Beclin-1)、微管相关蛋白1的轻链3B(microtubule associated protein light chain 3B,LC3B)和泛素结合蛋白62(sequestosome 1,p62)的影响。方法:采用大脑中动脉闭塞法建立小鼠脑缺血再灌注损伤模型。将造模成功的小鼠按随机数字表法分成模型组及益脑康低(1 g/kg)、中(3 g/kg)、高(9 g/kg)剂量组,每组6只,假手术组和模型组灌胃等体积生理盐水,益脑康各剂量组连续灌胃给药3天,每天2次。给药结束后观察各组小鼠神经行为学评分;采用2,3,5-氯化三苯基四氮唑染色法观察脑梗死体积;采用实时荧光定量聚合酶链式法检测Beclin-1 mRNA,LC3B mRNA和p62 mRNA表达;采用酶联免疫吸附试验检测Beclin-1,LC3B和p62含量。结果:与假手术组比较,模型组小鼠缺血区域体积、神经损伤程度、Beclin-1和LC3B表达均增加(P<0.01),p62及其mRNA表达降低(P<0.01);与模型组比较,益脑康各剂量组小鼠缺血区域体积、神经损伤程度、Beclin-1和LC3B表达均降低(P<0.05),p62表达增加(P<0.05)。结论:益脑康胶囊对缺血再灌注模型小鼠自噬进程具有一定减缓作用,能减少Beclin-1和LC3B表达,增加p62表达。Objective:To study the effects of Yinaokang capsules on autophagy-related factors Beclin-1,LC3B and p62 in mice model of ischemia-reperfusion injury(IRI).Methods:IRI mice models were established using middle cerebral artery occlusion method.Mice models after successful modeling were divided into the model group,low(1 g/kg),moderate(3 g/kg)and high(9 g/kg)doses groups of Yinaokang capsules using random number table method,with six in each group,sham operation group and the model group were drenched with equal volume of saline,different dose groups of Yinaokang capsules were administered for three consecutive days by gavage,twice each day.By the end of medication,to observe neurobehavioral scores of the mice in different groups;2,3,5-Triphenyltetrazolium chloride staining method was used to observe the volume of cerebral infarction;real-time fluorescence quantitative polymerase chain method was adopted to detect the expressions of Beclin-1 mRNA,LC3B mRNA and p62 mRNA;ELISA to measure the contents of Beclin-1,LC3B and p62.Results:Compared with sham operation group,volume ratio of ischemic area,degree of nerve damage,the expressions of Beclin-1 and LC3B increased in the model group(P<0.01),the expression of p62 and p62 mRNA reduced(P<0.01);compared with the model group,volume ratio of ischemic area,degree of nerve damage,the expressions of Beclin-1 and LC3B lowered in different doses groups of Yinaokang capsules(P<0.05),the expressions of p62 increased(P<0.05).Conclusion:Yinaokang capsule has a certain effects on slowing down the autophagy process of IRI mice,manifested by reducing the expressions of Beclin-1,LC3B,and increasing the expressions of p62.

关 键 词：脑卒中 缺血再灌注 自噬 益脑康 

分 类 号：R34[医药卫生—基础医学]