基于不同时间点观察针刺对脑缺血再灌注损伤大鼠海马组织Beclin-1及Caspase-3表达的影响  

Effects of acupuncture on Beclin-1 and Caspase-3 expression in hippocampal tissue of rats with cerebral ischemia-reperfusion injury:Observations at different time points

作　　者：卢小叶 郑慧娥 吕倩忆 李棋龙 张泓[1] 田浩梅[1] Xiao-ye LU;Hui-e ZHENG;Qian-yi Lu;Qi-Iong LI;Hong ZHANG;Hao-mei TIAN(College of Acupuncture and Massage,Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410007

出　　处：《World Journal of Acupuncture-Moxibustion》2023年第3期228-236,共9页世界针灸杂志（英文版）

基　　金：Sponsored by National Natural Science Foundation of China:81874508;Hunan Provincial Natural Science Foundation:2020JJ4065;Natural Science Foundation of Changsha Municipal Science and Technology Bureau:kq2014094;Innovation Platform Open Foundation of Hunan Provincial Education Department:19K068;University Student Innovation and Entrepreneurship Training Program of Higher Education Division of Ministry of Education of China:201910541050;Open Foundation of Hunan University of Chinese Medicine:2018YXJS01。

摘　　要：Objective:To observe the changes in the neurological score,infarct size,and autophagy-and apoptosisrelated indicators following the acupuncture treatment of rats with cerebral ischemia-reperfusion injury(CIRI) in order to investigate the effects of acupuncture on autophagy and apoptosis in the hippocampal tissue of CIRI rats Methods:In this study,170 Sprague-Dawley rats were randomly assigned to the normal group(n=34),sham group(n=34),and model establishment group(n=102).A CIRI rat model was established in the model establishment group.The rats of the model establishment group were then randomly and equally allocated to the middle cerebral artery occlusion/reperfusion group(MCAO/R group),acupuncture group(AC group),and edaravone group(ED group)(n=34 each).Each group was further subdivided into the 24-h and 72-h subgroups(n=17 each).The rats of the MCAO/R group were immobilized for 30 min and administered with an intraperitoneal injection of 0.9% sodium chloride solution.The rats of the AC group were immobilized,and they received acupuncture at the "Dazhui(GV 14)","Shuigou(GV 26)",and" Baihui(GV 20)" and an intraperitoneal injection of 0.9% sodium chloride solution,with the acupuncture needles left in place for 30 min.The rats of the ED group were immobilized for 30min and administered with an intraperitoneal injection of edaravone(5 mg/kg).All interventions were performed once every 12 h.Investigations of the neurological score,infarct size using triphenyl tetrazolium chloride staining,hippocampal Caspase-3 protein expression by lmmunohistochemical assay,hippocampal Beclin-1 expression by Western blotting,autophagosomes in the hippocampus by transmission electron microscopy,and apoptotic rate in the brain tissue of the ischemic side using the terminal deoxynucleotidyl transferase dUTP nick end labeling assay were performed both before and after the interventions.Results:Before intervention,the neurological score of the model establishment group was significantly lower than that of the normal and sham groups(P<0.0

关 键 词：ACUPUNCTURE Cerebral ischemia-reperfusion injury BECLIN-1 Caspase-3 

分 类 号：R245[医药卫生—针灸推拿学]