长蛇灸对肾阳虚大鼠HPA轴及肝脏11β-HSD1的影响  

Effects of long-snake moxibustion on the HPA axis and liver 11β-HSD1 expression in rats with kidney yang deficiency

作　　者：胡秀武[1,2,3] 黄辉 唐丽梅 耿乐乐[1,2,3] 邓陈英 黄平 曾景娇 Xiu-wu HU;Hui HUANG;Li-mei TANG;Le-le GENG;Chen-ying DENG;Ping HUANG;Jing-jiao ZENG(Hongdu Hospital of Traditional Chinese Medicine,Nanchang 330006,China;Jiangxi Clinical Research Center of Acupuncture and Moxibustion Medicine,Nanchang 330006,China;Nanchang mayor Key Laboratory of Snake Moxibustion Effect Mechanism and Governor Vessel Specificity,Nanchang 330006,China)

机构地区：[1]南昌市洪都中医院,南昌330006 [2]江西省针灸医学临床研究中心,南昌330006 [3]南昌市长蛇灸效应机制和督脉特异性重点实验室,南昌330006

出　　处：《World Journal of Acupuncture-Moxibustion》2023年第3期237-243,共7页世界针灸杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China:81960900。

摘　　要：Objective:To observe the effects of long-snake moxibustion on the hypothalamic-pituitary-adrenocortical(HPA)axis and hepatic 11β-hydroxy steroid dehydrogenase type 1(11β-HSD1)expression in rats with kidney yang deficiency to provide a basis for later in-depth exploration of the action mechanism of longsnakemoxibustion on suchrats.Methods:Fifteen SPF-grade,male,SD rats were randomly divided into a blank control group,a model group,and a long-snake moxibustion treatment group,with five rats in each group.Hydrocortisone powder(30 mg/kg)was administered by gavage at a volume of 10 mL/kg to prepare the rat model of kidney yang deficiency.After successful modeling,the rats in the long-snake moxibustion treatment group underwent long-snake moxibustion treatment every other day along the governor vessel from Dazhui(GV14)to Shenshu(BL23),for a period of 14 days.The remaining two groups were secured in the same way as the long-snake moxibustion treatment group,although they did not receive any treatment.The body weight,rectal temperature,and spontaneous activity count of the rats,as well as serum levels of corticotropin releasing hormone(CRH)and corticosterone(CORT)were detected by ELISA before modeling,after modeling,and after treatment.The amount of 11β-HSD1 protein in rat liver was determined by immunohistochemistry and Western blot analysis.Results:Compared with the rats in the blank control group,those in the model group exhibited a significant decrease in the trend of body weight growth and in rectal temperature(P<0.05),as well as a slight yet non-significant decrease in spontaneous activity count(P>0.05);compared with the rats in the model group,the rats in the treatment group exhibited a significant increase in rectal temperature(P<0.05)and in spontaneous activity count(P<0.05).Moreover,after 14 days of treatment,compared with the rats in the blank,the rats in the model group exhibited a significant decrease in serum cORT content(P<0.05)and in the expression of 11β-HSD1 in the liver(P<0.05),as well as a slight

关 键 词：11β-HSD1 Long-snake moxibustion Kidney yang deficiency syndrome HPA axis 

分 类 号：R245[医药卫生—针灸推拿学]