头针改善丙戊酸钠诱导的孤独症大鼠行为学及其机制研究  

Scalp acupuncture improves disordered behavior in the valproic acid-induced rat model of autism

作　　者：黄龙生 葛品[2] 康洁[3] 刘桂华[2] 洪钰 王静蓉 陈楚安 庄婉玉 郭敬民[2] 冯叶彬[2] 张宏 欧萍[2] Long-sheng HUANG;Pin GE;Jie KANG;Gui-hua LIU;Yu HONG;Jing-rong WANG;Chu-an CHEN;Wan-yu ZHUANG;Jing-min GUO;Ye-bin FENG;Hong ZHANG;Ping OU(Clinical Medical College of Acupuncture Moxibustion and Rehabilitation,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Fujian Maternity and Child Health Hospital,College of Clinical Medicine for Obstetrics&Gynecology and Pediatrics,Fujian Medical University,Fuzhou 350001,China;Department of TCM Syndrome Research Base,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]广州中医药大学针灸康复临床医学院,广州510006 [2]福建医科大学妇儿临床医学院,福州350001 [3]福建中医药大学中医证研究基地,福州350122

出　　处：《World Journal of Acupuncture-Moxibustion》2023年第3期252-261,共10页世界针灸杂志（英文版）

基　　金：Supported by the Fujian Provincial Medical Innovation Project:2020Y9145;Fujian Provincial Department of Science and Technology:2020J01335,2022J01363;Fujian University of Traditional Chinese Medicine Project:X2021023 and X2021024;Research Foundation of Fujian Provincial Maternity and Child Health Hospital:YCXM20-28and YCXM20-07。

摘　　要：Objectives:To investigate the underlying mechanisms of scalp acupuncture treatment(SAT)on autism spectrum disorder(ASD).Methods:Thirty male Wistar rat pups that had been prenatally exposed to valproic acid sodium(VPA)were randomly divided into the VPA,VPA+acupoint,and VPA+non-acupoint groups using the random number table method,with 10 rats in each group.Ten pups who had been prenatally exposed to saline were assigned to the control group(CG).There was no intervention in either the control or VPA groups.In the acupoint group,"Shenting(GV24),"bilateral"Benshen(GB13)"were manipulated.In the non-acupoint group,the area below the costal space was stimulated.Acupuncture stimulation lasted for 40 min,with manual twisting of the needles every 10 min,5 days/week,with 2 days of rest per week,for a total duration of 4 weeks.After the corresponding treatments,behavioral tests(including the open field,social interaction,and Morris water maze tests)were performed to evaluate the therapeutic effects.RT-PCR and Western blotting were performed to detect the expression of neuregulin 1(NRG1)/ErbB4.Results:In the open field test,the activity time spent in the central area in the VPA+acupoint group was significantly longer than that in the VPA group and VPA+non-acupoint group(both P<0.05).The total length in the VPA+acupoint group was significantly longer than that in the VPA group(P<0.05).The number of bouts in the central area of the VPA+acupoint group was significantly higher than that of the VPA group(P<0.05).In session I of social interaction test,all experimental rats spent more time interacting with stranger 1(all P<0.05).In session Il,the CG and VPA+acupoint groups rats showed more interest in searching for new strangers,but the VPA+non-acupoint group spent more time interacting with stranger 1 than with stranger 2(all P<0.05).In the Morris water maze test,compared with the VPA group,the latency of the VPA+acupoint group was shorter(day 2,3,4,5,P<0.05);compared with VPA+acupoint group,the latency of the VPA+non-acupoint grou

关 键 词：Autism spectrum disorder ACUPUNCTURE NRG1-ErbB4 Social behavior Morris water maze 

分 类 号：R245[医药卫生—针灸推拿学]