基于PACAP-cAMP信号通路研究电针治疗骶上脊髓损伤后逼尿肌反射亢进型膀胱的效应机制  

Underlying mechnism of electroacupuncture for treating detrusor hyperreflexia after suprasacral spinal cord injury through the PACAP-cAMP signaling pathway

作　　者：许明[1] 刘琼[1] 邓石峰 刘继生[1] 李亚 焦子远 匡静之[1] 艾坤[1] 张泓[1] Ming XU;Qiong LIU;Shi-feng DENG;Ji-sheng LIU;Ya LI;Zi-yuan JIAO;Jing-zhi KUANG;Kun AI;Hong ZHANG(School of Acupuncture-Moxibustion and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,长沙410208

出　　处：《World Journal of Acupuncture-Moxibustion》2023年第3期273-281,共9页世界针灸杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China:82274666,82205255;Natural Science Foundation of Hunan Province:2022JJ30036,2022JJ40312,20221140301;Research Project of Education Department of Hunan Province:20C1432,21B0369;Discipline of Integrated Traditional Chinese and Western Medicine of Hunan Province:2020ZXYJH23。

摘　　要：Objective:To elucidate the underlying mechanism and effect of electroacupuncture(EA)on the neurogenic bladder following suprasacral spinal cord injury(ssCI).A rat model of detrusor hyperreflexia after SsCI was established to examine the urodynamics,detrusor muscle tissue morphology,the protein and mRNA expression levels of pituitary adenylate cyclase activating peptide(PACAP)and its receptor PAC1R,and cyclic adenosine monophosphate(cAMP)content in the detrusor muscle with a focus on the PACAPcAMP signaling pathway.Method:A total of 72 female SD rats were randomized into control group and sham operation group(n=12 per group)by using a random number table.The remaining 48 rats were established into the model of detrusor hyperreflexia after SsCI.After successful modeling,these rats were randomly assigned to model,EA,and EA+PACAP6-38 groups(n=12 per group).The unsuccessful modeled rats were used for exploratory observation.For the rats in EA group,"Ciliao(BL32)""Zhongji(CV3)",and"Sanyinjiao(SP6)"were needled and stimulated by EA.The PACAP receptor antagonist PACAP6-38 was administered intraperitoneally in the EA+PACAP6-38 group before EA,and EA was applied for seven consecutive days.After treatment,the urodynamics of the rats were analyzed,and hematoxylin and eosin staining was used to examine rat bladder detrusor tissue morphology.The expressions of PACAP-38 and PAC1R were detected by immunohistochemistry and Western blot.The mRNA expression levels of PACAP-38 and PAC1R were examined by RT-qPCR,while cAMP content was detected by ELISA.Results:(1)Compared with sham operation group,it was exhibited disarray in the transitional epithelium cells of the bladder in the modeled rats.The intercellular space was significantly widened,accompanied by inflammatory cell infiltration and noticeable tissue edema.Both the bladder initial pressure and leak point pressure of the rats were higher(P<0.01),whereas the maximum cystometric capacity and bladder compliance were lower(P<0.01).The protein and mRNA expression levels of PACAP-3

关 键 词：ELECTROACUPUNCTURE Neurogenic bladder Suprasacral spinal cord injury(SscI) URODYNAMICS Pituitary adenylate cyclase activating peptide(PACAP) Cyclic adenosine monophosphate(cAMP) 

分 类 号：R245[医药卫生—针灸推拿学]