不同保存条件对血清样本细胞因子测定的影响  

Effects of different storage conditions on determination of cytokines in serum samples

作　　者：毛镭篥[1] 张丽[1] 李薇[1] 黄媛[1] 王斐[1] 吴卫[1] MAO Leili;ZHANG Li;LI Wei;HUANG Yuan;WANG Fei;WU Wei(Department of Clinical Laboratory,Chinese Academy of Medical Science&Peking Union Medical College Hospital,Beijing 100730,China)

机构地区：[1]中国医学科学院北京协和医院检验科,北京100730

出　　处：《临床检验杂志》2023年第6期411-416,共6页Chinese Journal of Clinical Laboratory Science

摘　　要：目的探讨保存温度、保存时间对血清样本中白介素6(IL-6)、白介素8(IL-8)、白介素10(IL-10)、肿瘤坏死因子α(TNF-α)测定的影响。方法随机选取北京协和医院就诊的120例患者,根据保存条件分为5组:室温原管组40例,室温原管保存;室温分装组20例,血清分至EP管室温保存;4℃原管组20例,4℃保存不分装;4℃分装组20例,血清分至EP管4℃保存;-20℃组20例,血清分至EP管-20℃保存。室温原管组中的20例标本和室温分装组、4℃原管组、4℃分装组分别测定即刻、6 h、24 h的IL-6、IL-8、IL-10、TNF-α;室温原管组中的另20例标本用于分析IL-8的稳定性,分别测定即刻、2 h、3 h、4 h、5 h的IL-8;-20℃组测定即刻及-20℃冷冻6个月后的IL-6、IL-8、IL-10、TNF-α。结果室温原管组中IL-6、IL-10、TNF-α在6 h、24 h结果与即刻相比,差异均无统计学意义,IL-8在6 h、24 h结果与即刻相比,差异均有统计学意义(P<0.05),比对通过率分别为5%、0%,假阳性率分别为60%、70%。室温放置2 h、3 h、4 h、5 h IL-8的比对通过率分别为80%、35%、15%、10%,P值分别为0.820、0.461、0.076、0.003,仅2 h的结果通过稳定性比对。虽然其他4组各时间点细胞因子的测定结果与即刻相比差异均无统计学意义,但室温分装组IL-8在6 h、24 h的比对通过率分别为65%、50%;4℃原管组的IL-8在24 h的比对通过率为55%,均未通过比对。只有4℃分装组和-20℃组细胞因子各时间点的测定结果与即刻相比,差异均无统计学意义,且各项目比对通过率均≥80%,结果稳定可靠。结论采用血清样本联合检测IL-6、IL-8、IL-10、TNF-α时,分析前时间控制在2 h内,可以采取室温原管放置;2~24 h内推荐4℃分装;-20℃时6个月结果稳定。各实验室若不能满足2 h内及时上机检测,为避免IL-8的假阳性,建议分装冻存。Objective To explore the influences of temperature and time in storage on the determination for interleukin-6(IL-6),interleukin-8(IL-8),interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)in serum samples.Methods A total of 120 serum samples from patients were randomly selected from Peking Union Medical College Hospital and divided into 5 groups according to storage conditions as follows:room temperature original tube group:40 serum samples were stored in original tubes at room temperature;room temperature subpackage group:20 samples were divided into EP tubes and stored at room temperature;4℃C original tube group:20 samples in original tubes were stored at 4℃C without subpacage;4 C subpackage group:20 samples were divided into EP tubes and stored at 4℃;-20℃group:20 samples were divided into EP tubes and stored at-20℃.The levels of IL-6,IL-8,IL-10 and TNF-αin 20 samples from the each group:room temperature original tubes,room temperature subpackage tubes,4 C original tubes and 4℃subpackage tubes were measured immediately,6 hours and 24 hours,respectively.The levels of IL-8 of the serum samples in the other 20 tubes stored in room temperature were measured immediately,2,3,4 and 5 hours respectively to analyze the stability of test results.The levels of IL-6,IL-8,IL-10 and TNF-αin the samples stored at-20 C group were tested immediately and after freezing for 6 months respectively.Results For the samples of room temperature original tube group,the results of IL-6,IL-10 and TNF-αmeasured at 6 h and 24 h do not showed statistically significant different compared with those measured immediately,but the results of IL-8 measured at 6 and 24 hours were both significantly different from the results measured immediately(P<0.05).The comparisonable pass rate were 5%and 0%,and the false positive rate were 60%and 70%,respectively.The comparisonable pass rates of the results of IL-8 measured after 2,3,4 and 5 hours at room temperature were 80%,35%,15%and 10%,respectively with the P values of 0.820,0.461,0.07

关 键 词：细胞因子 血清样本 温度 时间 保存条件 

分 类 号：R446[医药卫生—诊断学]