Bromocriptine protects perilesional spinal cord neurons from lipotoxicity after spinal cord injury  

作　　者：Ying Peng Zhuoxuan Li Zhiyang Zhang Yinglun Chen Renyuan Wang Nixi Xu Yuanwu Cao Chang Jiang Zixian Chen Haodong Lin 

机构地区：[1]Trauma Center,Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,China [2]Department of Orthopedics,Zhongshan Hospital,Fudan University,Shanghai,China [3]Department of Rehabilitation Medicine,Shanghai Geriatric Medical Center,Shanghai,China

出　　处：《Neural Regeneration Research》2024年第5期1142-1149,共8页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,Nos.82071376(to ZC)and 82001471(to CJ);the Natural Science Foundation of Shanghai,No.20ZR1410500(to ZC).

摘　　要：Recent studies have revealed that lipid droplets accumulate in neurons after brain injury and evoke lipotoxicity,damaging the neurons.However,how lipids are metabolized by spinal cord neurons after spinal cord injury remains unclear.Herein,we investigated lipid metabolism by spinal cord neurons after spinal cord injury and identified lipid-lowering compounds to treat spinal cord injury.We found that lipid droplets accumulated in perilesional spinal cord neurons after spinal cord injury in mice.Lipid droplet accumulation could be induced by myelin debris in HT22 cells.Myelin debris degradation by phospholipase led to massive free fatty acid production,which increased lipid droplet synthesis,β-oxidation,and oxidative phosphorylation.Excessive oxidative phosphorylation increased reactive oxygen species generation,which led to increased lipid peroxidation and HT22 cell apoptosis.Bromocriptine was identified as a lipid-lowering compound that inhibited phosphorylation of cytosolic phospholipase A2 by reducing the phosphorylation of extracellular signal-regulated kinases 1/2 in the mitogen-activated protein kinase pathway,thereby inhibiting myelin debris degradation by cytosolic phospholipase A2 and alleviating lipid droplet accumulation in myelin debris-treated HT22 cells.Motor function,lipid droplet accumulation in spinal cord neurons and neuronal survival were all improved in bromocriptine-treated mice after spinal cord injury.The results suggest that bromocriptine can protect neurons from lipotoxic damage after spinal cord injury via the extracellular signal-regulated kinases 1/2-cytosolic phospholipase A2 pathway.

关 键 词：BROMOCRIPTINE central nervous system cytosolic phospholipase A2 high-content screening lipid droplet lipid metabolism LIPOTOXICITY mitogen-activated protein kinase spinal cord injury spinal cord neurons 

分 类 号：R651.2[医药卫生—外科学]