支气管哮喘合并肺炎支原体感染患者血清致炎细胞因子水平及对TLR4/NF-κB信号通路的影响  

Serum inflammatory cytokines and their effect on TLR4/NF-B signaling pathway in patients with bronchial asthma complicated with Mycoplasma pneumoniae infection

作　　者：陈冉 曹雯雯 宋争昌 刘欣 刘圆圆 韩倩 安霞 李文鑫 孟玲 CHEN Ran;CAO Wen-wen;SONG Zheng-chang;LIU Xin;LIU Yuan-yuan;HAN Qian;AN Xia;LI Wen-xin;MENG Ling(The Second Affiliated Hospital of Shandong First Medical University,Tai'an,Shandong 271099,China)

机构地区：[1]山东第一医科大学第二附属医院呼吸与危重症医学科,山东泰安271099

出　　处：《中华医院感染学杂志》2023年第15期2271-2275,共5页Chinese Journal of Nosocomiology

基　　金：山东省医药卫生科技发展计划基金资助项目(2018ws118)。

摘　　要：目的 探讨支气管哮喘合并肺炎支原体感染患者血清致炎细胞因子水平变化及对Toll-样受体4/核因子-κB(TLR4/NF-κB)信号通路的影响。方法 选取2020年3月-2022年9月山东第一医科大学第二附属医院收治的98例支气管哮喘合并肺炎支原体感染的患者为感染组,同期106例支气管哮喘未合并肺炎支原体感染的患者为未感染组,另选113名健康体检者作为对照组。比较三组致炎细胞因子、TLR4、NF-κB、NF-κB抑制蛋白(IκB)信使核糖核酸(mRNA)相对表达量、TLR4蛋白、NF-κB蛋白、磷酸化NF-κB抑制蛋白(p-IκB)及不同肺炎支原体抗体(MP-IgM)滴度致炎细胞因子水平。结果 感染组血清致炎细胞因子水平高于未感染组、对照组,未感染组高于对照组(P<0.05);高滴度组、中滴度组血清致炎细胞因子水平均高于低滴度组(P<0.05);感染组TLR4、NF-κB mRNA相对表达量、TLR4蛋白、NF-κB蛋白、p-IκB蛋白均高于未感染组、对照组,未感染组高于对照组,感染组IκB mRNA相对表达量低于未感染组、对照组,未感染组低于对照组(P<0.05)。结论 血清致炎细胞因子在支气管哮喘合并肺炎支原体感染患者MP-IgM抗体中、高滴度中呈高表达,上述因子与TLR4、NF-κB mRNA相对表达量、TLR4蛋白、NF-κB蛋白、p-IκB蛋白随着支气管哮喘、肺炎支原体感染的发生而升高,IκB mRNA相对表达量随之下降。OBJECTIVE To investigate the changes of serum inflammatory cytokines and their effect on toll-like receptor 4/nuclear factor-kB(TLR4/NF-kB)signaling pathway in patients with bronchial asthma complicated with Mycoplasma pneumoniae infection.METHODS From Mar 2020 to Sep 2022,98 patients with bronchial asthma complicated with Mycoplasma pneumoniae infection admitted to the Second Affiliated Hospital of Shandong First Medical University were enrolled as the infection group,and 106 patients without Mycoplasma pneumoniae infection were recruited in the uninfected group.Another ll3 healthy people were selected as the control group.The inflammatory cytokines,relative expression of TLR4,NF-kB,inhibitor of NF-sB(IkB)messenger ribonucleic acid(mRNA),TLR4 protein,NF-B protein,phosphorylated NF-kB inhibitor protein(p-IB)and inflammatory cytokines in patients with different MP-IgM titers among the three groups were compared.RESULTS The levels of serum inflammatory cytokines in the infection group were higher than that in the uninfected group and the control group,and that in the uninfected group was higher than in the control group(P<0.05).The levels of serum inflammatory cytokines in the high titer group and the medium titer group were higher than those in the low titer group(P<0.05).The relative expression of TLR4,NF-B mRNA,TLR4 protein,NF-kB protein,p-IsB protein in the infection group was higher than those in the uninfected group and the control group,and those in the uninfected group was higher than those in the control group.The relative mRNA expression level of IB in the infection group was lower than that in the uninfected group and the control group,and that in the uninfected group was lower than in the control group(P<0.05).CONCLUSION Serum inflammatory cytokines were highly expressed in MP-IgM medium and high titer groups of patients with bronchial asthma complicated with Mycoplasma pneumoniae infection.The above indexes and the relative expression levels of TLR4,NF-kB mRNA,TLR4 protein,NF-kB protein and p-IkB prote

关 键 词：支气管哮喘 肺炎支原体感染 致炎细胞因子 Toll-样受体4/核因子-κB信号通路 

分 类 号：R562.25[医药卫生—呼吸系统]