升清胶囊有效组分对氧化损伤肝细胞CYP7A1及SCP2的影响  

Effects of Effective Components of Shengqing Capsule(升清胶囊)on CYP7A1 and SCP2 in Oxidative Damaged Hepatocytes

作　　者：李阳 张静喆[2] 顾宏刚[2] LI Yang;ZHANG Jingzhe;GU Honggang(People's Hospital of Zhengzhou,People's Hospital of Henan University of Chinese Medicine,Zhengzhou 450003,Henan,China;Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]郑州人民医院,河南中医药大学人民医院,河南郑州450003 [2]上海中医药大学附属龙华医院,上海200032

出　　处：《辽宁中医杂志》2023年第8期188-192,I0005,共6页Liaoning Journal of Traditional Chinese Medicine

基　　金：上海市科委自然科学基金项目(09ZR1432300)。

摘　　要：目的观察升清胶囊有效组分对过氧化氢(H2O2)诱导的人肝细胞LO2的氧化损伤模型中胆固醇7α-羟化酶(CYP7A1)和固醇携带蛋白2(SCP2)mRNA及蛋白表达的影响。方法将正常人LO2肝细胞分为正常组、模型组、对照组(维生素E)及治疗组(升清胶囊有效组分)。模型组、对照组及治疗组选用H2O2诱导人肝细胞LO2氧化损伤模型;通过检测细胞外乳酸脱氢酶(LDH)和细胞内谷胱甘肽(GSH)来分析各组对H2O2造成人肝细胞的氧化损伤的影响;治疗组、对照组每孔分别加入2000μL含有升清胶囊有效组分、维生素E的RPMI-1640培养液,正常组和模型组加入等体积的RPMI-1640培养液,培养24 h。采用实时荧光定量PCR和Western Blot检测CYP7A1和SCP2的mRNA和蛋白表达。结果(1)相对于模型组,治疗组、对照组LDH含量下降(P<0.05),GSH含量明显升高(P<0.01);相对于对照组,治疗组LDH含量下降(P<0.05),GSH含量稍上升(P>0.05)。(2)在实时荧光定量PCR中,相对于模型组(100%),正常组、治疗组和对照组肝细胞CYP7A1 mRNA的表达上调(分别为296.16%、242.65%、126.61%),相对于对照组(100%),治疗组肝细胞CYP7A1 mRNA的表达上调(为191.65%);相对于模型组(100%),正常组、治疗组和对照组肝细胞SCP2 mRNA的表达下调(分别为15.40%、33.41%、39.94%),相对于对照组(100%),治疗组肝细胞SCP2 mRNA的表达下调(为83.66%)。(3)Western Blot检测中,正常组、治疗组和对照组肝细胞CYP7A1蛋白表达均明显高于模型组(P<0.01),治疗组CYP7A1蛋白表达稍高于对照组,差异无统计学意义(P>0.05);正常组肝细胞SCP2蛋白表达明显低于模型组(P<0.01),治疗组、对照组SCP2蛋白表达低于模型组(P<0.05),治疗组略微低于对照组,差异无统计学意义(P>0.05)。结论升清胶囊有效组分可以降低肝细胞的氧化损伤,修复肝细胞内胆固醇的正常代谢,提高CYP7A1 mRNA和蛋白量的表达,降低SCP2 mRNA和蛋白量的表达,减少成石性胆汁的形成,其�Objective To observe the effect of the effective components of Shengqing Capsule(升清胶囊)on the expressions of cholesterol 7α-hydroxylase(CYP7A1)and sterol carrying protein 2(SCP2)mRNA and protein in the oxidative damage model of human hepatocyte LO2 induced by H2O2.Methods Normal human LO2 hepatocytes were divided into normal group,model group,control group(vitamin E)and treatment group(effective components of Shengqing Capsule).In model group,control group and treatment group,H2O2 was used to induce the formation of normal human hepatocyte LO2 oxidative damage model.The effects of each group on H2O2-induced oxidative damage of human hepatocytes were analyzed by detecting extracellular lactic dehydrogenase(LDH)and intracellular glutathione(GSH).In treatment group and control group,2000μL RPMI-1640 culture medium containing effective component combination of Shengqing Capsule or vitamin E was added into each pore respectively,and in normal group and model group,RPMI-1640 culture medium of equal volume was added for 24 hours.The mRNA and protein expressions of CYP7A1 and SCP2 were detected by real-time PCR and Western blot.Results(1)Compared with those of the model group,the content of LDH in the treatment group and the control group decreased(P<0.05),the content of GSH increased significantly(P<0.01).Compared with those of the control group,the content of LDH in the treatment group decreased(P<0.05),while the content of GSH increased slightly(P>0.05).(2)Compared with that of the model group(100%),the expression of CYP7A1 mRNA in normal group,treatment group and control group was up-regulated(296.16%,242.65%and 126.61%,respectively),and the expression of CYP7A1 mRNA in treatment group was up-regulated(191.65%)compared with that of the control group(100%).Compared with that of the model group(100%),the expression of SCP2 mRNA in normal group,treatment group and control group was down-regulated(15.40%,33.41%and 39.94%,respectively).Compared with that of the control group(100%),the expression of SCP2 mRNA in t

关 键 词：氧化损伤 胆固醇结石 升清胶囊 胆固醇7Α-羟化酶 固醇携带蛋白2 

分 类 号：R285[医药卫生—中药学]