多重PCR检测技术在儿童社区获得性肺炎病原学诊断中的临床应用价值  

CLINICAL VALUE OF MULTIPLEX PCR IN ETIOLOGICAL DIAGNOSIS OF COMMUNITY ACQUIRED PNEUMONIA IN CHILDREN

作　　者：常颖 黄光举[1] 张慧玉[1] 李娟[1] 李佳[1] 徐静 CHANG Ying;HUANG Guangju;ZHANG Huiyu;LI Juan;LI Jia(Tangshan malternal and child health hospital,Tangshan 063000,China)

机构地区：[1]河北省唐山市妇幼保健院,063000

出　　处：《中国煤炭工业医学杂志》2023年第3期299-302,共4页Chinese Journal of Coal Industry Medicine

基　　金：河北省2018年度医学科研究重点课题计划(编号:20181334)。

摘　　要：目的探讨多重PCR在儿童社区获得性肺炎病原学诊断中的价值。方法随机抽取2022年6月1日—2022年11月30日该院呼吸科住院的社区获得性肺炎患儿共计100例,入院后留取痰标本,行痰培养、深部痰多重PCR、血清肺炎支原体抗体及衣原体抗体检测。结果多重PCR细菌检测的阳性率为46%,痰培养阳性率为17%,两组间差异有统计学意义(χ^(2)=19.488,P<0.01),多重PCR检测的肺炎支原体阳性率为81%,血清肺炎支原体抗体阳性率为73%,两组间差异无统计学意义(χ^(2)=1.807,P=0.179),多重PCR检测的肺炎衣原体阳性率为8%,血清肺炎衣原体抗体阳性率为4%,两组间异无统计学意义(χ^(2)=1.418,P=0.234),多重PCR与痰培养、血清学检测肺炎支原体抗体、衣原体抗体的结果均具有较好一致性。结论多重PCR在儿童社区获得性肺炎病原诊断方面具有快速、灵敏、特异性高的特点,可为临床医生合理使用抗生素提供依据。Objective To investigate the value of multiplex PCR in the etiological diagnosis of community-acquired pneumonia in children.Methods A total of one hundred children with community-acquired pneumonia who were hospitalized in the respiratory department of our hospital from June 1,2022 to November 30,2022 were enrolled,and sputum samples were retained after admission,and sputum culture,deep sputum multiplex PCR,serum Mycoplasma pneumoniae antibody and chlamydia antibody were detected.Results The positive rate of multiplex PCR bacterial detection was 46%,the positive rate of sputum culture was 17%,the difference between the two groups was statistically significant(χ^(2)=19.488,P<0.01),the positive rate of Mycoplasma pneumoniae by multiplex PCR was 81%,the positive rate of M.pneumoniae antibody was 73%,there was no significant difference between the two groups(χ^(2)=1.807,P=0.179),the positive rate of Chlamydia pneumoniae by multiplex PCR was 8%,and the positive rate of serum Chlamydia pneumoniae antibody was 4%.There was no significant difference between the two groups(χ^(2)=1.418,P=0.234),and the results of multiplex PCR,sputum culture,serological detection of Mycoplasma pneumoniae antibody and chlamydia antibody were well consistent.Conclusion Multiplex PCR has the characteristics of rapid,sensitive and highly specific in the diagnosis of community-acquired pneumonia pathogens in children,which can provide a basis for clinicians to rationally use antibiotics.

关 键 词：社区获得性肺炎病原学诊断 多重PCR检测 痰培养 肺炎支原体抗体 衣原体抗体 

分 类 号：R725.6[医药卫生—儿科]