广金钱草中黄酮及黄酮苷类化学成分研究  

Study on flavonoids and flavonoid glycosides from Desmodium styracifolium

作　　者：吴云秋 闫炳雄 阮丽君 姚彩云[1] 刘喜慧 宋梦新 黄媛 宋志军[1] WU Yun-qiu;YAN Bing-xiong;RUAN Li-jun;YAO Cai-yun;LIU Xi-hui;SONG Meng-xin;HUANG Yuan;SONG Zhi-jun(National Engineering Research center for Southwestern Endangered Medicinal Materials Resources Development,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China;Pharmaceutical College,Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西壮族自治区药用植物园,西南濒危药材资源开发国家工程实验室,广西南宁530023 [2]广西医科大学药学院,广西南宁530021

出　　处：《中草药》2023年第15期4780-4788,共9页Chinese Traditional and Herbal Drugs

基　　金：中国科学院科技服务网络计划(STS计划)区域重点项目A类(KFJ-STS-QYZD-2021-03-004);广西壮族自治区中医药管理局自筹经费课题(GXZYA20220003);广西中医药适宜技术开发与推广项目(GZSY22-03);广西药用植物保育人才小高地自主研究项目(GXYYXGD202203)。

摘　　要：目的对广金钱草Desmodium styracifolium中的黄酮及黄酮苷类化学成分进行研究。方法采用溶剂提取法、MCI HP20柱色谱、硅胶柱色谱、Sephadex LH-20凝胶柱色谱、ODS柱色谱、制备高效液相色谱等方法进行分离纯化,并利用现代波谱技术对化合物进行结构鉴定。结果从广金钱草醇提取物醋酸乙酯和正丁醇部位中分离得到31个黄酮及黄酮苷类化合物,分别鉴定为5,7-二羟基-2’-甲氧基-3’,4’-亚甲二氧基二氢异黄酮(1)、5,7-二羟基-2’,4’-二甲氧基二氢异黄酮(2)、金圣草黄素(3)、异鼠李素(4)、红车轴草素(5)、parvisoflavanone(6)、3,3’-二甲氧基槲皮素(7)、5,7,3’-三羟基-4’,5’-二甲氧基异黄酮(8)、5,7-二羟基-3’,4’-二甲氧基黄酮(9)、eriocoumaronochromone(10)、染料木素(11)、苜蓿素(12)、3’-甲氧基大豆苷元(13)、5,7,2’-三羟基-4’-甲氧基二氢异黄酮(14)、山柰酚(15)、木犀草素(16)、大豆苷元(17)、7,3’-二羟基-4’,5’-二甲氧基异黄酮(18)、spicatolignanB(19)、槲皮素(20)、香豌豆酚(21)、3-甲氧基山柰酚(22)、芹菜素(23)、二氢山柰酚(24)、dalbergiodin(25)、2’-羟基染料木素(26)、木犀草苷(27)、异荭草苷(28)、染料木素-7-O-β-D-呋喃芹糖基-(1→6)-O-β-D-吡喃葡萄糖苷(29)、异牡荆苷(30)、维采宁-2(31)。结论化合物6~8、10、14、18~19为首次从广金钱草中分离得到,丰富了广金钱草黄酮类成分的结构类型。Objective To study the flavonoids and flavonoid glycosides of Desmodium styracifolium.Methods The chemical constituents were isolated and purified by solvent extraction,MCI HP20,silica gel,Sephadex LH-20,ODS and preparative high performance liquid chromatography.Their structures were identified by spectral data.Results Thirty-one flavonoids and flavonoid glycosides were isolated from the ethyl acetate and n-butanol parts of the ethanol extract of the whole plant of D.styracifolium and identified as 5,7-dihydroxy-2'-methoxy-3',4'-methylenedioxyisoflavanone(1),5,7-dihydroxy-2',4'-dimethoxyisoflavanone(2),chrysoeriol(3),isorhamntin(4),pratensein(5),parvisoflavanone(6),3,3'-dimethoxyquercetin(7),5,7,3'-trihydroxy-4',5'-dimethoxyisoflavone(8),5,7-dihydroxy-3',4'-dimethoxyflavone(9),eriocoumaronochromone(10),genistein(11),tricin(12),3'-methoxydaidzein(13),ferreirin(14),kaempferol(15),luteolin(16),daidzein(17),7,3'-dihydroxy-4',5'-dimethoxyisoflavone(18),spicatolignan B(19),quercetin(20),orobol(21),3-methoxy kaempferol(22),apigenin(23),dihydrokaempferol(24),dalbergiodin(25),2'-hydroxygenistein(26),luteolin 7-O-β-D-glucoside(27),isoorientin(28),ambocin(29),isovitexin(30),and vicenin II(31).Conclusion Compounds 6-8,10,14,and 18-19 are isolated from D.styracifolium for the first time,which can enrich the structure types of flavonoids in D.styracifolium.

关 键 词：广金钱草 黄酮 黄酮苷 3 3’-二甲氧基槲皮素 5 7 2’-三羟基-4’-甲氧基二氢异黄酮 parvisoflavanone spicatolignan B 

分 类 号：R284.1[医药卫生—中药学]