衣原体遗传操作的研究进展  

Advances in genetic manipulation of Chlamydiae

作　　者：李舒怡 陈超群 LI Shuyi;CHEN Chaoqun(Department of Medical Immunology,Institute of Pathogenic Biology,Hengyang Medical School of University of South China,Hengyang 421001,Hunan,China)

机构地区：[1]南华大学衡阳医学院病原生物学研究所医学免疫学教研室,湖南衡阳421001

出　　处：《中国病原生物学杂志》2023年第10期1226-1230,共5页Journal of Pathogen Biology

基　　金：湖南省教育厅创新平台开放基金项目(No.18k072)。

摘　　要：衣原体(Chlamydiae)感染可引起人和动物的多种疾病。长期以来,衣原体固有的细胞内周期和使用抗生素作为可选择标记的限制阻碍了对该病原体进行基因操作分子工具的发展。然而,最近各类遗传操作工具的发展使我们对改变衣原体基因组的能力有了显著提高,这将加速阐明这种顽固性病原体的生物学和发病机制。本综述中阐述了衣原体遗传操作的挑战和可行性分析,以及其相关分子遗传工具的进展。Chlamydiae infection can cause a variety of diseases in humans and animals.For a long time,the inherent intracellular cycle of chlamydia and the limitations of using antibiotics as selectable markers have hindered the develop-ment of molecular tools for gene manipulation of this pathogen.However,the recent development of various genetic ma-nipulation tools has significantly improved our ability to alter the chlamydia genome,which will accelerate the elucidating of the biology and pathogenesis of this stubborn pathogen.In this review,we discuss the challenges and feasibility of ge-netic manipulation of chlamydia,as well as the development of related molecular genetic tools.

关 键 词：衣原体 遗传操作 分子遗传工具 综述 

分 类 号：R375[医药卫生—病原生物学]