血清PDGF、FGF-2水平与膝骨关节炎病情严重程度的相关性  

The correlation between serum levels of PDGF and FGF-2 and the severity of knee osteoarthritis

作　　者：梁治权[1] 孟庆才[1] 李祥生 邓迎杰[1] 刘鹏程[1] Liang Zhiquan;Meng Qingcai;Li Xiangsheng;Deng Yingjie;Liu Pengcheng(The Second Department of Orthopedics,Traditional Chinese Medicine Hospital of Xinjiang Uygur Autonomous Region,Xinjiang Province,Urumqi 830000,China)

机构地区：[1]新疆医科大学第四附属医院骨二科,830000

出　　处：《疑难病杂志》2023年第9期972-976,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：新疆维吾尔自治区自然科学基金(2020D01C143)。

摘　　要：目的探讨血清血小板衍生生长因子(PDGF)、成纤维细胞生长因子2(FGF-2)水平与膝骨关节炎患者病情严重程度的相关性。方法选取2020年7月—2023年1月新疆医科大学第四附属医院骨二科诊治膝骨关节炎患者105例作为关节炎组,依据严重程度分为轻中度亚组62例和重度亚组43例;同时纳入健康体检者102例作为健康对照组。采用PCR法检测PDGF基因rs974819位点、FGF-2基因rs2922979位点多态性并进行测序鉴定;采用双抗体夹心酶联免疫吸附法检测血清PDGF水平,酶联免疫吸附法检测血清FGF-2水平;多因素Logistic回归分析影响膝骨关节炎患者病情严重程度的因素。结果健康对照组和关节炎组PDGF基因rs974819位点、FGF-2基因rs2922979位点上均检出GG型、GC型、CC型3种基因型,关节炎组携带CC型基因型及C等位基因占比显著高于健康对照组(PDGF:χ^(2)/P=41.726/<0.001,54.382/<0.001;FGF-2:χ^(2)/P=65.987/<0.001,88.988/<0.001),C等位基因可能是膝骨关节炎的易感基因。关节炎组血清PDGF、FGF-2水平低于健康对照组(t/P=11.715/<0.001、22.079/<0.001)。重度亚组血清PDGF、FGF-2水平低于轻中度亚组(t/P=6.525/<0.001、8.304/<0.001),IL-17、TNF-α水平高于轻中度亚组(t/P=6.032/<0.001、8.952/<0.001)。血清PDGF、FGF-2高是膝骨关节炎患者病情进展为重度的保护因素[OR(95%CI)=0.849(0.753~0.957)、0.834(0.747~0.931)]。结论随膝骨关节炎患者疾病严重程度增加,血清PDGF、FGF-2水平降低,PDGF基因rs974819位点、FGF-2基因rs2922979位点的C等位基因可能是膝骨关节炎的易感基因。Objective To investigate the correlation between serum levels of platelet derived growth factor(PDGF)and fibroblast growth factor 2(FGF-2)and the severity of knee osteoarthritis in patients.Methods One hundred and five patients with knee osteoarthritis diagnosed and treated by the Second Department of Orthopedics at Xinjiang Uygur Autonomous Region Traditional Chinese Medicine Hospital from July 2020 to January 2023 were selected as the arthritis group.They were divided into mild to moderate subgroups of 62 cases and severe subgroups of 43 cases based on their severity;102 health examinees were included as a healthy control group.PCR was used to detect the polymorphism of PDGF gene rs974819 and FGF-2 gene rs2922979,and sequencing was performed for identification;Double antibody sandwich enzyme-linked immunosorbent assay was used to detect serum PDGF levels,and enzyme-linked immunosorbent assay was used to detect serum FGF-2 levels;Multiple logistic regression analysis of factors affecting the severity of knee osteoarthritis in patients.Results The GG genotype,GC genotype,and CC genotype were detected at rs974819 of the PDGF gene and rs2922979 of the FGF-2 gene in both the healthy control group and the arthritis group.The proportion of CC genotype and C allele carried by the arthritis group was significantly higher than that of the healthy control group(PDGF:χ^(2)/P=41.726/<0.001,54.382/<0.001;FGF-2:χ^(2)/P=65.987/<0.001,88.988/<0.001),the C allele may be a susceptibility gene for knee osteoarthritis.The serum levels of PDGF and FGF-2 in the arthritis group were lower than those in the healthy control group(t/P=11.715/<0.001,22.079/<0.001).The serum levels of PDGF and FGF-2 in the severe subgroup were lower than those in the mild to moderate subgroup(t/P=6.525/<0.001,8.304/<0.001),IL-17,TNF-αThe levels were higher than those in the mild to moderate subgroups(t/P=6.032/<0.001,8.952/<0.001).High levels of serum PDGF and FGF-2 are protective factors for the progression of knee osteoarthritis patients to severe sev

关 键 词：膝骨关节炎 血小板衍生生长因子 成纤维细胞生长因子2 病情程度 

分 类 号：R684.3[医药卫生—骨科学]