特应性皮炎患儿血浆外泌体LncRNA MIR155HG表达与病情严重程度的相关性  

The correlation between the expression of plasma exosomes LncRNA MIR155HG and the severity of atopic dermatitis in children

作　　者：刘岩[1] 凌琬茗[1] 赵瑞雪 王浩 Liu Yan;Ling Wanming;Zhao Ruixue;Wang Hao(Department of Dermatology,Hebei Provincial Children's Hospital,Hebei Province,Shijiazhuang 050000,China)

机构地区：[1]河北省儿童医院皮肤科,石家庄050000

出　　处：《疑难病杂志》2023年第9期977-981,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省医学科学研究课题计划项目(20211788)。

摘　　要：目的检测血浆外泌体长链非编码RNA(LncRNA)MIR155HG在特应性皮炎(AD)患儿中的表达并分析其与病情严重程度的关系。方法选择2021年12月—2023年1月河北省儿童医院皮肤科收治的AD患儿118例为AD组,同期医院体检健康儿童120例为健康对照组。分离受试儿童血浆外泌体并进行鉴定,检测血浆外泌体LncRNA MIR155HG表达水平。比较不同病情程度AD患儿血浆外泌体LncRNA MIR155HG表达水平及AD积分指数(SCORAD)评分;Pearson法分析AD患儿血浆外泌体LncRNA MIR155HG与SCORAD评分相关性;受试者工作特征曲线(ROC)分析血浆外泌体LncRNA MIR155HG表达水平对中度、重度AD的诊断价值。结果与健康对照组比较,AD组患儿血浆外泌体LncRNA MIR155HG表达水平显著升高(t/P=23.843/<0.001);轻度亚组、中度亚组、重度亚组AD患儿血浆外泌体LncRNA MIR155HG表达水平及SCORAD评分依次升高(F/P=41.773/<0.001、260.201/<0.001);AD患儿血浆外泌体LncRNA MIR155HG表达水平与SCORAD评分呈正相关(r/P=0.673/<0.001);ROC曲线显示,血浆外泌体LncRNA MIR155HG表达水平诊断中重度AD的曲线下面积(AUC)分别为0.831、0.802,诊断中度和重度AD的AUC比较差异无统计学意义(Z/P=1.008/0.313)。结论AD患儿血浆外泌体LncRNA MIR155HG高表达,且其可能与AD病情的严重程度有关,对AD严重程度有较高诊断价值,临床监测血浆外泌体LncRNA MIR155HG水平变化可能对靶向防治AD患儿有重要意义。Objective To detect the expression of long chain non-coding RNA(LncRNA)MIR155HG in plasma exosomes in children with atopic dermatitis(AD)and analyze its relationship with the severity of the disease.Methods From December 2021 to January 2023,118 children with AD in the Dermatology of Hebei Children's Hospital were selected as the AD group,and 120 healthy children in the same period were selected as the healthy control group.Isolate and identify plasma exosomes from the tested children,and detect the expression level of plasma exosomes LncRNA MIR155HG.Compare the expression levels of plasma extracellular vesicle LncRNA MIR155HG and the score of AD score(SCORAD)in children with different degrees of AD.Pearson method was used to analyze the correlation between plasma extracellular vesicle LncRNA MIR155HG and SCORAD score in children with AD.The diagnostic value of analyzing the expression level of LncRNA MIR155HG in plasma exosomes using the receiver operating characteristic curve(ROC)for moderate to severe AD.Results Compared with the healthy control group,the expression level of plasma extracellular vesicle LncRNA MIR155HG in the AD group was significantly increased(t/P=23.843/<0.001).The expression level of plasma extracellular vesicle LncRNA MIR155HG and SCORAD score in mild,moderate,and severe subgroups of AD patients increased sequentially(F/P=41.773/<0.001,260.201/<0.001).The expression level of plasma extracellular vesicle LncRNA MIR155HG in children with AD was positively correlated with SCORAD score(r/P=0.673/<0.001).The ROC curve showed that the area under the curve(AUC)for diagnosing moderate to severe AD was 0.831 and 0.802,respectively,based on the expression level of LncRNA MIR155HG in plasma exosomes.There was no statistically significant difference in AUC diagnosis between moderate and severe AD(Z/P=1.008/0.313).Conclusions The high expression of plasma extracellular vesicle LncRNA MIR155HG in children with Alzheimer's disease may be related to the severity of the disease,and has high diagnostic val

关 键 词：特应性皮炎 血浆外泌体 长链非编码RNA MIR155HG 病情严重程度 

分 类 号：R758.3[医药卫生—皮肤病学与性病学]