Cdc42蛋白参与中性粒细胞弹性蛋白酶诱导的人气道上皮细胞黏蛋白高分泌  

Cdc42 protein participates in neutrophil Elastase induced Mucoprotein hypersecretion in human airway epithelial cells

作　　者：何明欣 周向东[2] 杨雅楼 徐立[2] 张华[3] 李琪[2] He Mingxin;Zhou Xiangdong;Yang Yalou;Xu Li;Zhang Hua;Li Qi(Department of Respiratory Medicine,the First Affiliated Hospital of Hainan Medical College,Hainan Province,Haikou 570102,China)

机构地区：[1]北京大学深圳医院,深圳518036 [2]海南医学院第一附属医院呼吸内科,海口570102 [3]海南医学院,海口571199

出　　处：《疑难病杂志》2023年第9期982-986,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：国家自然科学基金资助项目(81860001、82160012、82260001);科技部重大专项(2022YFC2305004);海南省重点研发项目(ZDYF2020223);海南省自然科学基金项目(822MS071);海南省重点研发计划国际科技合作项目(GHYF2022011);海南省重大科技专项(ZDKJ202004、ZDKJ2021036、ZDKJ2021038);海南省创新团队项目(820CXTD448);国家卫生健康委员会热带病防治重点实验室开放课题(2021NHCTDCKFKT21008);中国医学科学院医学与健康科技创新工程项目(2019-12M-5-023);海南医学院校级教学成果奖培育项目(HYjcpx202202)。

摘　　要：目的探讨细胞分裂周期蛋白42(Cdc42)与中性粒细胞弹性蛋白酶(NE)诱导的气道上皮细胞黏蛋白(MUC)5AC高分泌的关系。方法体外培养人气道上皮细胞(16HBE),以Cdc42 siRNA和NE进行干预,将细胞分为6组:对照组、NE组、NE+Cdc42 siRNA组、Cdc42 siRNA组、阴性siRNA组、NE+阴性siRNA组。采用Western blot法检测Cdc42蛋白的相对含量,ELISA和免疫荧光检测各组细胞MUC5AC蛋白的表达水平。结果与对照组相比,NE组Cdc42蛋白含量明显增加(P<0.05),细胞MUC5AC蛋白表达水平亦显著增高(P<0.001);与NE组比较,NE+Cdc42 siRNA组的MUC5AC蛋白水平显著降低(P<0.05),其Cdc42蛋白含量亦明显降低(P<0.001);Cdc42 siRNA组以及阴性siRNA组的Cdc42蛋白、MUC5AC蛋白含量相较于对照组变化不大(P>0.05)。结论Cdc42在NE诱导的气道上皮细胞黏液高分泌的形成过程中可能发挥着重要的介导作用。Objective To investigate the relationship between cell division Cyclin 42(Cdc42)and neutrophil Elastase(NE)induced hypersecretion of Mucoprotein(MUC)5AC in airway epithelial cells.Methods Human airway epithelial cells(16HBE)were cultured in vitro and intervened with Cdc42 siRNA and NE.The cells were divided into six groups:control group,NE group,NE+Cdc42 siRNA group,Cdc42 siRNA group,negative siRNA group,and NE+negative siRNA group.Western blot method was used to detect the relative content of Cdc42 protein,and ELISA and immunofluorescence were used to detect the expression level of MUC5AC protein in each group of cells.Results Compared with the control group,the content of Cdc42 protein in the NE group was significantly increased(P<0.05),and the expression level of MUC5AC protein in cells was also significantly increased(P<0.001);Compared with the NE group,the MUC5AC protein level in the NE+Cdc42 siRNA group was significantly reduced(P<0.05),and the Cdc42 protein content was also significantly reduced(P<0.05);The MUC5AC protein content in the Cdc42 siRNA group and the negative siRNA group showed little change compared to the control group(P>0.05).Conclusion Cdc42 may play an important mediating role in the formation of NE induced mucus hypersecretion in airway epithelial cells.

关 键 词：气道黏蛋白5AC 细胞分裂周期蛋白42 中性粒细胞弹性蛋白酶 黏液高分泌 

分 类 号：R332.2[医药卫生—人体生理学]