基于高效指纹图谱和网络药理学的琥珀消石颗粒质量标志物预测  

Q-marker Prediction of Hupo Xiaoshi Granules Based on High-efficiency Fingerprinting and Network Pharmacology

作　　者：赵琦 李海松[1] 闫菲 王继升[1] 米新宝 赵聪 张华南 李曰庆[1] ZHAO Qi;LI Haisong;YAN Fei;WANG Jisheng;MI Xinbao;ZHAO Cong;ZHANG Huanan;LI Yueqing(Department of Andrology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Department of Dermatology,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院男科,北京100700 [2]北京中医药大学东直门医院皮科,北京100700

出　　处：《世界中医药》2023年第16期2273-2279,共7页World Chinese Medicine

基　　金：国家自然科学基金面上项目(82174385);中华中医药学会青年求实项目(2021-QNQS-03)。

摘　　要：目的:基于高效指纹图谱和网络药理学预测琥珀消石颗粒(HXG)中质量标志物(Q-Marker)。方法:构建HXG的高效液相色谱(HPLC)指纹图谱检测手段,指认共有峰和归属;应用网络药理学技术构建HXG的“成分-靶点-通路”网络图,预测其潜在Q-Marker。结果:HXG的指纹图谱结果共指认出5个共有峰;网络药理学确定了5种关键组成、35个潜在核心靶点和6条重要通路,初步预测槲皮素-3-O-(2G-α-L-鼠李糖基)-芸香糖苷、山柰酚-3-O-芸香糖苷、β-蜕皮甾酮、槲皮素、山柰素为HXG潜在的Q-Marker。结论:通过高效指纹图谱和网络药理学分析预测了HXG中的Q-Marker,方法科学并且可重复,为HXG的质量标准提供依据,也为今后系统研究其作用机制提供研究基础。Objective:To predict the quality markers(Q-markers)of Hupo Xiaoshi Granules(HXG)based on high-efficiency fingerprinting and network pharmacology.Methods:An high-performance liquid chromatography(HPLC)fingerprinting method was established to identify and assign the common peaks in HXG.Network pharmacology was applied to construct a“component-target-pathway”network to predict potential Q-markers in HXG.Results:The fingerprinting results of HXG identified five common peaks.The network pharmacology analysis revealed five key components,35 potential core targets,and six important pathways in HXG.Based on this analysis,the potential Q-markers of HXG were preliminarily predicted to be quercetin-3-O-(2G-α-L-rhamnosyl)-rutinoside,kaempferol-3-O-rutinoside,β-ecdysterone,quercetin,and kaempferol.Conclusion:The prediction of Q-markers in HXG using high-efficiency fingerprinting and network pharmacology analysis is a scientific and reproducible method,which can provide a basis for establishing the quality standards of HXG and serve as research foundation for future research on its mechanisms of action.

关 键 词：琥珀消石颗粒 高效指纹图谱 网络药理学 质量标志物 槲皮素-3-O-(2G-α-L-鼠李糖基)-芸香糖苷 β-蜕皮甾酮 抗炎 抗氧化 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]