右美托咪啶对重度烧伤SD大鼠NGAL和TNF-α的影响  

The effects of Dexmedetomidine on NGAL and TNF-α in severely burned SD rats

作　　者：徐琳[1] 万昊[2] 罗佛全[1] 龚红燕 XU Lin;WAN hao;LUO Foquan(The First Affiliated Hospital of Nanchang University,Anesthesiology Department of Anesthesia and Pain Medical Center,Nanchang,330006;The First Affiliated Hospital of Nanchang University,General Surgery department,Nanchang,330006)

机构地区：[1]南昌大学第一附属医院麻醉与疼痛医学中心麻醉科,南昌330006 [2]南昌大学第一附属医院普外科,南昌330006

出　　处：《江西医药》2023年第7期786-787,798,共3页Jiangxi Medical Journal

基　　金：江西省卫生计生委普通科技计划项目,编号20203220。

摘　　要：目的探讨右美托咪啶对重度烧伤SD大鼠中性粒细胞明胶酶相关脂质运载蛋白(NGAL)和肿瘤坏死因子(TNF-α)的影响。方法研究将30只SD大鼠分为右美托咪啶组(D组)、烧伤组(B组)和空白对照组(C组)。所有大鼠建立30%体表面积烧伤模型,D组大鼠腹腔注射右美托咪啶30 ug/kg,B组给与等剂量盐水。采用ELISA法测定NGAL、TNF-α水平,HE染色观察肾组织结构改变。结果与C组相比,B组和D组大鼠肾组织出现炎性细胞浸润,肾小管肿胀伴上皮变性坏死,NGAL以及TNF-α的表达均上升。与B组相比,D组大鼠肾组织病理改变明显减轻,NGAL、TNF-α的表达均下降。结论右美托咪啶可降低重度烧伤SD大鼠血清中NGAL和TNF-α的水平,具有一定的肾保护作用。Objective To study the effect of dexmedetomidine on neutrophil gelatinase-associated lipid carrier protein(NGAL)and tumor necrosis factor(TNF-α) in severely burned SD rats.Methods Thirty SD rats were divided into dexmedetomidine group(group D),burn group(group B) and blank control group(group C).All rats were established with 30% body surface area burn model.Group D rats were injected with 30 ug/kg of dexmedetomidine intraperitoneally,and group B rats were given the same dose of saline.Determination of NGAL and TNF-α by ELISA.HE staining was used to observe the changes of renal tissue structure.Results Compared with group C,rats in groups B and D showed inflammatory cell infiltration,swelling of renal tubules with epithelial degeneration and necrosis.The expression of NGAL and TNF-α were found increasedin in group B and D.Compared with group B,the pathological changes of renal tissue in group D were significantly reduced.The expression of NGAL,TNF-α were decreased.Conclusion Dextrmetomidine can reduce NGAL and TNF in serum of SD rats with severe burns,it has certain renal protective effect.

关 键 词：右美托咪定 中性粒细胞明胶酶相关脂质运载蛋白 肿瘤坏死因子 

分 类 号：R644[医药卫生—外科学]