多基因聚合改良杂交稻恢复系福恢676的稻瘟病抗性  

Blast resistance improvement of hybrid rice restorer line Fuhui 676 by polygene polymerization

作　　者：朱永生[1,2] 蔡秋华 官华忠[3] 魏毅东 陈丽萍 谢华安[1,2] 陈志伟 张建福[1,2] Yongsheng Zhu;Qiuhua Cai;Huazhong Guan;Yidong Wei;Liping Chen;Huaan Xie;Zhiwei Chen;Jianfu Zhang(Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350018,China;Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China,Ministry of Agriculture and Rural/Fuzhou Branch,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences&Technology/Base of South-China,National Key Laboratory of Hybrid Rice/National Rice Engineering Laboratory of China,Fuzhou 350003,China;College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350007,China)

机构地区：[1]福建省农业科学院水稻研究所,福州350018 [2]农业农村部华南杂交水稻种质创新与分子育种重点实验室/国家水稻改良中心福州分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/福建省作物种质创新与分子育种省部共建国家重点实验室培育基地/杂交水稻国家重点实验室华南研究基地/水稻国家工程实验室,福州350003 [3]福建农林大学农学院,福州350007

出　　处：《科学通报》2023年第21期2812-2823,共12页Chinese Science Bulletin

基　　金：省属公益类科研院所基本科研项目(2020R1023002);福建省科技重大专项(2022NZ030014);“5511”协同创新工程项目农业种质资源圃(库)(XTCXGC2021019-SDS01)资助。

摘　　要：福恢676是一个综合性状优良但稻瘟病抗性不强的杂交水稻恢复系,该父本已测配育成的18个杂交稻品种通过了省级以上品种审定.为了改良其稻瘟病抗性,延长该恢复系在生产上的应用期限.以携带3个稻瘟病抗性基因(Pi-1、Pi-9和Pi-k^(h))的优质恢复系金恢1059为供体亲本,以福恢676为轮回亲本,通过回交导入、分子标记辅助选择及田间稻瘟病抗性鉴定相结合的方法,选育出5个抗病的稳定株系,其中3个株系聚合了Pi-1、Pi-9和Pi-kh.对这5个株系的综合农艺性状、品质和配合力等进行系统比较,并结合稻瘟病抗性鉴定.结果表明,与福恢676相比,改良株系及其与广8A、荃9311A和广占63-4S等不育系配制的杂种F1均表现出稻瘟病抗性明显增强,而其中株系6综合表现最优,遗传背景恢复率为97.1%,且其生育期、株高等主要农艺性状与福恢676测配组合无显著差异,但产量有所提高,品质得到改善.改良的株系6保留了福恢676产量高、恢复力强、配合力好等优良特点,品质有所提升,具有较好的应用前景.Rice is the staple food for more than half of the world's population.The success of hybrid rice in China has made outstanding contributions to global food security.Restorer line is one of the parents of hybrid rice,and its characteristics often determine the quality of a hybrid rice variety.Fuhui 676,as a restorer line parent of hybrid rice with high yield,good combining ability and excellent comprehensive characters,has been tested and bred 18 new hybrid rice varieties,which have passed the variety examination and approval at the provincial level or above.One of the varieties has been identified as a super rice variety by the Ministry of Agriculture and Rural Affairs,which with excellent performance in production.However,with the expansion of the extension area and the extension of time,the combination of the male parent was susceptible to rice blast.Studies have shown that breeding and application of disease-resistant varieties is one of the most economical and effective methods to control diseases.Long-term practice has also proved that aggregating multiple blast resistance genes with different resistance spectra into the same variety is an effective measure to cultivate varieties with durable resistance.Therefore,in order to effectively improve the blast resistance of the restorer line Fuhui 676,extend the application period of the restorer line in production and improve the efficiency of breeding,the cross was made between the high-quality restorer line Jinhui 1059 with three broad-spectrum rice blast resistance genes(Pi-1,Pi-9 and Pi-kh) bred by molecular marker-assisted selection and the susceptible restorer line Fuhui 676.Meanwhile,through the combination of backcross introductionbetween the restorer line Jinhui 1059 as the donor parent and the restorer line Fuhui 676 as the recurrent parent,molecular marker-assisted selection and identification of rice blast resistance in the field,five stable resistant lines were selected,of which three lines aggregated the rice blast resistance gene Pi-1,Pi-9 and Pi-kh

关 键 词：稻瘟病抗性 基因聚合 遗传改良 分子标记 配合力 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]