濒危植物粗茎紫金牛茎段愈伤组织再生体系的建立  

Establishment of Regeneration System of Stem Callus for Ardisia gi-gantifolia,a Rare and Endangered Species

作　　者：周艳 李冬梅[1] 吴坤林[1] 宁祖林[1] ZHOU Yan;LI Dongmei;WU Kunlin;NING Zulin(South China Botanical Garden,Chinese Academy of Sciences,Guangzhou,Guangdong 510650,China;Guizhou Botanical Garden,Guiyang,Guizhou 550001,China)

机构地区：[1]中国科学院华南植物园,广东广州510650 [2]贵州省植物园,贵州贵阳550001

出　　处：《热带作物学报》2023年第8期1588-1595,共8页Chinese Journal of Tropical Crops

基　　金：广东省自然科学基金项目(No.2022A1515010904);广东省重点领域研发计划项目(No.2022B1111040003)。

摘　　要：粗茎紫金牛(Ardisia gigantifolia Stapf)原产于云南河口县,野生个体数量稀少,被列为极危物种,其叶、花、果均具较好的观赏性,具有重要的保护价值和较好的园林开发应用前景。本研究以粗茎紫金牛茎段为外植体建立其高效愈伤组织再生体系。结果表明:最佳愈伤组织诱导培养基为WPM+0.50 mg/L TDZ+0.10 mg/L NAA,诱导率为96.67%;愈伤组织在培养基WPM+0.50 mg/L TDZ中增殖系数达4.42,在培养基WPM+2.00 mg/L 6-BA+0.20 mg/L NAA中不定芽诱导率为100%;不定芽在培养基WPM+4.00 mg/L 6-BA中增殖系数达3.90,于培养基WPM+10%CW+0.20 mg/L NAA中生根率为100%,且不定根发达,植株生长健壮;组培苗移栽至珍珠岩∶泥炭土=1∶3的混合基质中,60 d后成活率达96.67%。该研究可为种苗的规模化生产提供技术支撑,也为该物种的保护、种苗繁育和分子生物学研究提供理论基础和技术支持。Ardisia gigantifolia Stapf is native to Hekou county,Yunnan province,with few wild individuals,and is listed as a critically endangered species.It has important protection value and good prospect of garden development and ap-plication.The stem segments of aseptic seedlings of A.gigantifolia were used as the explants to establish a propagation in vitro.The results showed that the optimal medium for callus induction was WPM+0.50 mg/L TDZ+0.10 mg/L NAA,and the induction rate of callus was 96.67%.The optional medium for callus proliferation was WPM+0.50 mg/L TDZ and the multiplication coefficient was 4.42.The optimal medium for callus differentiation was WPM+2.00 mg/L 6-BA+0.20 mg/L NAA,and the induction rate reached 100%.The optional medium for adventitious bud proliferation culture was WPM+4.00 mg/L 6-BA,the multiplication coefficient was 3.90,and the cluster buds grew well.The op-tional rooting medium was WPM+10%coconut water+0.20 mg/L NAA,the rooting rate reached 100%,the root system developed well and the test-tube plantlets grew vigorously.Plantlets were transplanted into the mixture substrate with the volume ratio of perlite and peat soil of 1∶3,the survival rate was 96.67%after 60 days and plants grew well.This study can provide technical support for the large-scale seedling production of A.gigantifolia,as well as theoretical basis and technical support for the protection,seedling breeding,and molecular biology research of this species.

关 键 词：濒危植物 粗茎紫金牛 愈伤组织 再生体系 

分 类 号：S688.9[农业科学—观赏园艺]